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PDI Inhibitor Screening Assay Kit

Cat.No. : Kit-0671
Product Overview : PDI Inhibitor Screening Assay Kit provides a simple, homogenous assay for screening modulators of protein disulfide isomerase (PDI) enzymatic activity in a 96-well microplate. The basis for the assay is the PDI-catalyzed reduction of insulin in the presence of DTT, resulting in the formation of insulin aggregates which in turnn bind to the red-emitting fluorogenic PDI Detection Reagent. Relative to analogous turbidimetric assays of PDI activity, the fluorescence-based assay provides a vastly improved assay signal window, improved lower detection limit and superior Z"-score (>0.8). The kit includes human recombinant PID enzyme as positive control, as well as the PDI inhibitor bacitracin as a negative control. This kit will provide a quantitative readout of PDI enzymatic activity is a robust and HTP fashion and can be applied to identification of PDI inhibitors from chemical libraries.
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Size : 2 x 96 tests
Applications : Functional Studies more details

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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