"rFFAR1" Related Products

rFFAR1 U2OS β-Arrestin GPCR Assay Kit

Cat.No.: Kit-1506
Product Overview: β-Arrestin GPCR cells are engineered to co-the ProLink (PK) tagged GPCR and the Enzyme Acceptor (EA) tagged β-Arrestin. Activation of the GPCR-PK induces β-Arrestin-EA recruitment, forcing complementation of the two β-galactosidase enzyme fragments (EA and PK). The resulting functional enzyme hydrolyzes substrate to generate a chemiluminescent signal. These cells have been modified to prevent long term propagation and expansion using a proprietary compound that has no apparent effect on assay performance.
Size: 100 dp(1 x 96-well)/200 dp(2 x 96-well)/1000 dp(10 x 96-well)
Target Species: Rat
Storage: Short term (2 weeks): Store in vapor phase of liquid N2
Kit Components: 1.25 x 10^6 cells per vial in 0.1 mL (kit contains cells, detection reagents and plate(s))

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Optional requirements on this protein    +Expand
C-fusion    N-fusion   Non-tagged
His    GST   Fc   Others
<1.0 eu/μg    <0.1 eu/μg   <0.01 eu/μg   Not required
Monomer Isolation    Dimer Isolation    Not required
>80% by SDS-PAGE    >90% by SDS-PAGE   >95% by SDS-PAGE   Others

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