Active Recombinant Canine IL12B protein, His-tagged

• Cat.No.: IL12B-03C
• Product Overview: Recombinant Canine IL12B(Ile23-Ser329) fused with His tag at C-terminal was expressed in NS0.

Specification

• Source: Mammalian cells
• Species: Canine
• Tag: His
• Predicted N Terminal: Ile23
• Form: Lyophilized from a 0.2 μm filtered solution in PBS.
• Bio-activity: Measured by its ability to compete with biotinylated canine IL-12/IL-23 p40 for binding with immobilized rhIL-12 R beta 1/Fc Chimera in a functional ELISA assay.
• Molecular Mass: Predicted Molecular Mass: 36.3 (monomer) kDaSDS-PAGE: 43 kDa, reducing conditions
• Endotoxin: <0.10 EU per 1 μg of the protein by the LAL method.
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Storage: Avoid repeated freeze-thaw cycles.12 months from date of receipt, -20 to -70 centigrade as supplied.1 month, 2 to 8 centigrade under sterile conditions after reconstitution.3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
• Reconstitution: Reconstitute at 100 μg/mL in sterile PBS.

Gene Information

• Gene Name: IL12B interleukin 12B [ Canis lupus familiaris ]
• Official Symbol: IL12B
• Synonyms: CLMF p40; IL-12 subunit p40; IL-12B; cytotoxic lymphocyte maturation factor 40 kDa subunit; interleukin 12B (natural killer cell stimulatory factor 2, cytotoxic lymphocyte maturation factor 2, p40); interleukin-12 p40 subunit
• Gene ID: 403976
• mRNA Refseq: NM_001003292
• Protein Refseq: NP_001003292
• UniProt ID: Q28268
• Chromosome Location: chromosome: 4
• Pathway: African trypanosomiasis, organism-specific biosystem; Allograft rejection, conserved biosystem; Cytokine-cytokine receptor interaction, organism-specific biosystem
• Function: cytokine activity; interleukin-12 alpha subunit binding