Active Recombinant Canine NRG1 Protein

• Cat.No.: NRG1-1050C
• Product Overview: Recombinant canine NRG1 isoform alpha (XP_858147.1) extracellular domain (Ser19-Lys240) was expressed with five amino acids (DDDDK) at the C- terminus.

Specification

• Source: HEK293
• Species: Canine
• Predicted N Terminal: Ser 19
• Form: Lyophilized from sterile PBS, pH 7.4, 5%~8% trehalose and mannitol.
• Bio-activity: Measured by its binding ability in a functional ELISA. Immobilized canine NRG1 at 10 μg/ml (100 μl/well) can bind human ErbB4-Fc, The EC50 of human ErbB4-Fc is 0.19-0.43 μg/ml.
• Molecular Mass: The recombinant canine NRG1 comprises 228 amino acids and has a predicted molecular mass of 24.7 kDa. The apparent molecular mass of the protein is approximately 47 kDa in SDS-PAGE under reducing conditions due to glycosylation.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
• Purity: >95 % as determined by SDS-PAGE.
• Stability: Samples are stable for up to twelve months from date of receipt at -70ºC.
• Storage: Store it under sterile conditions at -20ºC~-70ºC. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.25 ug/ul. Centrifuge the vial at 4℃ before opening to recover the entire contents.

Gene Information

• Gene Name: NRG1 neuregulin 1 [ Canis lupus familiaris ]
• Official Symbol: NRG1
• Synonyms: pro-neuregulin-1, membrane-bound isoform
• Gene ID: 482861
• Chromosome Location: chromosome: 16
• Pathway: ARMS-mediated activation, organism-specific biosystem; Cytokine Signaling in Immune system, organism-specific biosystem; Downstream signal transduction, organism-specific biosystem