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Active Recombinant Cynomolgus CD226 protein, Fc-tagged

Cat.No. : CD226-248C
Product Overview : Recombinant Cynomolgus CD226(Glu19-Asn247) fused Fc region of Human IgG1 was expressed in HEK293.
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Description : DNAX accessory molecule-1 (DNAM-1), also known as CD226, is a 65 kDa type I transmembrane glycoprotein in the immunoglobulin superfamily. Mature cynomolgus DNAM-1 contains a 236 amino acid (aa) extracellular domain (ECD) with two Ig-like C2-set domains and a 61 aa cytoplasmic region that contains motifs for binding PDZ domains and band 4.1 family proteins. Within the ECD, cynomolgus DNAM-1 shares 93%, 53% and 50% aa sequence identity with human, mouse, and rat DNAM-1, respectively. DNAM-1 is expressed on multiple lymphoid, myeloid cells, and activated vascular endothelial cells and interacts with CD155/PVR and Nectin-2/CD112. It competes with CD96 and TIGIT for binding to these proteins. It associates in cis with Integrin beta 2/CD18 on activated, but not resting, NK, T, and mast cells. Ligation of DNAM-1 promotes the activation of NK cells, CD8+ T cells, and mast cells, Th17 polarization, dendritic cell maturation, megakaryocyte, and activated platelet adhesion to vascular endothelial cells, and monocyte extravasation; it also inhibits the formation of osteoclasts. Platelet-endothelium interactions mediated by DNAM-1 enable the metastasis of tumor cells to the lung. Downregulation of DNAM-1 on tumor cells can interfere with anti-tumor cellular immunity.
Source : HEK293
Species : Cynomolgus
Tag : Fc
Predicted N Terminal : Glu19
Form : Lyophilized from a 0.2 μm filtered solution in PBS.
Bio-activity : Measured by its binding ability in a functional ELISA. When Recombinant Cynomolgus Monkey DNAM-1/CD226 Fc Chimera is immobilized at 0.25 μg/mL (100 μL/well), the concentration of Recombinant Human CD155/PVR Fc Chimera that produces 50% of the optimal binding response is approximately 0.2-1 μg/mL.
Molecular Mass : Predicted Molecular Mass: 53 kDa
SDS-PAGE: 74-87 kDa, reducing conditions
Endotoxin : <0.1 EU per 1 μg of the protein by the LAL method.
Purity : >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Storage : Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 centigrade as supplied.
1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
Reconstitution : Reconstitute at 1 mg/mL in PBS.
Gene Name : CD226 CD226 molecule [ Macaca fascicularis ]
Official Symbol : CD226
Synonyms : CD226; CD226 molecule
Gene ID : 102117316

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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