|Product Overview:||Recombinant Cynomolgus Monkey TIMD4(Met22-Pro142) fused with Fc region of Human IgG1 at C-terminal was expressed in HEK293.|
|Description:||TIM-4 (T cell; immunoglobulin; mucin-4), also known as SMUCKLER, is an approximately 60 kDa member of the TIM family of immune regulating proteins. TIMs are type I transmembrane proteins with one Ig-like V domain and one Ser/Thr-rich mucin domain. Sequence alignment with the human TIM-4 shows that mature cynomolgus monkey TIM-4 has a predicted 280 aa extracellular domain (ECD), a 21 aa transmembrane segment and a 43 aa cytoplasmic tail. Within the ECD, cynomolgus monkey TIM-4 shares 87 % and 48 % aa sequence identity with human and mouse TIM-4, respectively. Structurally, TIM-4 is distinguished from other TIMs by the presence of an RGD motif in its Ig domain and the lack of a site for tyrosine phosphorylation in its cytoplasmic tail. The mucin domain in TIM-4 is larger than in TIM-1or TIM-3. TIM-4 is expressed by macrophages and mature dendritic cells but not by lymphocytes. TIM-4 binds specifically to TIM-1 which is also the cellular receptor for the hepatitis A virus, and has been implicated in the development of asthma. Among hematopoietic cells, TIM-1 is expressed on activated B and T cells, preferentially in the Th2 subset of CD4+ T cells. The interaction of TIM-4 with TIM-1 induces co-stimulatory and hyperproliferative signals in T cells.|
|Predicted N Terminal:||Glu25|
|Form:||Lyophilized from a 0.2 μm filtered solution in NaH2PO4, NaCl and EDTA.|
|Bio-activity:||Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated human T cells. The ED50 for this effect is 0.2-1.2 μg/mL.|
|Molecular Mass:||Predicted Molecular Mass: 57 kDa
SDS-PAGE: 95-112 kDa, reducing conditions
|Endotoxin:||<0.1 EU per 1 μg of the protein by the LAL method.|
|Purity:||>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.|
|Storage:||Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 centigrade as supplied.
1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
|Reconstitution:||Reconstitute at 500 μg/mL in PBS.|
|Gene Name:||TIMD4 T-cell immunoglobulin and mucin domain containing 4 [ Macaca fascicularis ]|
|Synonyms:||TIMD4; T-cell immunoglobulin and mucin domain containing 4|
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