Recombinant EV71 HEVAgp1 Protein, His&GST-tagged

• Cat.No.: HEVAgp1-807V
• Product Overview: Recombinant amino acids (Met 1-Gln 323) of human enterovirus 71 genome polyprotein (Q66478-1), corresponding to the protein VP0 precusor, a component of immature procapsids, was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.

Specification

• Source: Baculovirus-Insect Cells
• Species: EV71
• Tag: His&GST
• Predicted N Terminal: Met
• Form: Lyophilized from sterile 50mM Tris, 100mM NaCl, 2mM GSH, 0.5mM PMSF, pH 8.0, 5% ~ 8% trehalose and mannitol.
• Molecular Mass: The recombinant human enterovirus 71 protein VP0/GST chimera consists of 560 amino acids and has a calculated molecular mass of 63 kDa. It migrates as an approximately 58 kDa band in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
• Purity: >72 % as determined by SDS-PAGE
• Stability: Samples are stable for up to twelve months from date of receipt at -70ºC.
• Storage: Store it under sterile conditions at -20ºC~-70ºC. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.25 ug/ul. Centrifuge the vial at 4℃ before opening to recover the entire contents.

Gene Information

• Gene Name: HEVAgp1 genome polyprotein [ Enterovirus A ]
• Official Symbol: HEVAgp1
• Synonyms: genome polyprotein; VP4 (1A); VP2 (1B); VP3 (1C); VP1 (1D); protease 2A; protien 2B; protein 2C; protein 3A; 3B; protease 3C; 3D
• Gene ID: 1461111
• Protein Refseq: NP_042242
• UniProt ID: Q65900