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Active Recombinant Ferret TNF Protein

Cat.No. : TNF-1022F
Product Overview : Recombinant mature form of Ferret (Mustela putorius furo) TNFα (A3FBF1) (Val 77-Leu 233) was expressed, with an initial Met at the N-terminus.
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  • Gene Information
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Source : E. coli
Species : Ferret
Predicted N Terminal : Met
Form : Lyophilized from sterile PBS, pH 7.4, 5%~8% trehalose and mannitol.
Bio-activity : Measured in a cytotoxicity assay using L929 mouse fibrosarcoma cells in the presence of the metabolic inhibitor actinomycin D. The EC50 for this effect is typically 50-200 pg/ml.
Molecular Mass : The recombinant Ferret TNFα consists of 158 amino acids and has a calculated molecular mass of 17.3 kDa. The apparent molecular mass of the recombinant protein is approximately 16 kDa in SDS-PAGE under reducing conditions.
Endotoxin : < 1.0 EU per μg of the protein as determined by the LAL method.
Purity : >95 % as determined by SDS-PAGE.
Stability : Samples are stable for up to twelve months from date of receipt at -70ºC.
Storage : Store it under sterile conditions at -20ºC~-70ºC. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution : It is recommended that sterile water be added to the vial to prepare a stock solution of 0.25 ug/ul. Centrifuge the vial at 4℃ before opening to recover the entire contents.
Gene Name : TNF tumor necrosis factor [ Mustela putorius furo ]
Official Symbol : TNF
Synonyms : DIF; TNFA; TNF-a; TNFSF2; TNFalpha; TNF alpha; tumor necrosis factor alpha
Gene ID : 101687148
mRNA Refseq : NM_001310183
Protein Refseq : NP_001297112
MIM :
UniProt ID :

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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