Recombinant influenza A virus H5N1(A/common magpie/Hong Kong/5052/2007) HA protein(Met1-Gln530), His-tagged

• Cat.No.: HA-588V
• Product Overview: Recombinant influenza A virus (A/common magpie/Hong Kong/5052/2007(H5N1)) hemagglutinin (ACJ26242.1) (Met1-Gln530), termed as HA, was expressed in HEK293, fused with a polyhistidine tag at the C-terminus.

Specification

• Source: HEK293
• Species: H5N1
• Tag: C-His
• Protein length: Met1-Gln530
• Form: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Molecular Mass: The secreted recombinant HA subunit of influenza A virus (A/common magpie/Hong Kong/5052/2007(H5N1)) comprises 525 amino acids and has a predicted molecular mass of 60 kDa.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
• Purity: > 90 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.