Recombinant HIV gag Protein, His-tagged

• Cat.No.: gag-727V
• Product Overview: Recombinant HIV1 Gag-P24 (subtype D) (AAA44868.1) (Pro133-Leu363) was expressed with a polyhistidine tag at the C-terminus.

Specification

• Source: E. coli
• Species: HIV
• Tag: His
• Predicted N Terminal: His
• Form: Lyophilized from sterile PBS, pH 7.4, 5%~8% trehalose and mannitol.
• Molecular Mass: The recombinant HIV1 Gag-P24 (subtype D) consists of 238 amino acids and predicts a molecular mass of 26.6 kDa. It migrates as an approximately 27 kDa band in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
• Purity: >90 % as determined by SDS-PAGE.
• Stability: Samples are stable for up to twelve months from date of receipt at -70ºC.
• Storage: Store it under sterile conditions at -20ºC~-70ºC. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.25 ug/ul. Centrifuge the vial at 4℃ before opening to recover the entire contents.