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Recombinant HIV tat protein

Cat.No.: tat-190H
Product Overview: HIV-1 TAT Recombinant produced in E.coli is a single, non-glycosylated, polypeptide chain containing 101 amino acids encoded by two exons and having chain having a molecular mass of 21kDa.
Description: Human immunodeficiency virus type-1 (HIV-1) regulatory Tat protein plays an essential role in viral replication and infectivity. In addition, during acute infection, Tat is released extracellularly by infected cells and is taken up by neighboring cells where it transactivates viral replication and increases virus infectivity.
HIV-1 Tat activates transcription of HIV-1 viral genes by inducing phosphorylation of the C-terminal domain (CTD) of RNA polymerase II (RNAPII). Tat can also disturb cellular metabolism by inhibiting proliferation of antigen-specific T lymphocytes and by inducing cellular apoptosis. Tat-induced apoptosis of T-cells is attributed, in part, to the distortion of microtubules polymerization. LIS1 is a microtubule-associated protein that facilitates microtubule polymerization.
Source: E. coli
Species: HIV
Form: Sterile filtered colorless clear solution. 0.1% glycerol.
Molecular Mass: 21kDa
Protein length: 101 amino acids
Purity: Greater than 90.0% as determined by SDS-PAGE.
Usage: The products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
Stability: HIV-1 TAT Clade-C recombinant although stable at 4°C for 1 week, should be stored below -18°C. Please prevent freeze-thaw cycles.
Specificity: Immunoreactive with all sera of HIV-1 infected individuals.
Shipping: Ice Packs
Gene Name: tat p14 [ Human immunodeficiency virus 1 ]
Official Symbol: tat
Synonyms: tat; p14
Gene ID: 155871
Protein Refseq: NP_057853.1
UniProt ID: P04608

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Optional requirements on this protein    +Expand
C-fusion    N-fusion   Non-tagged
His    GST   Fc   Others
<1.0 eu/μg    <0.1 eu/μg   <0.01 eu/μg   Not required
Monomer Isolation    Dimer Isolation    Not required
>80% by SDS-PAGE    >90% by SDS-PAGE   >95% by SDS-PAGE   Others

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