Creative BioMart to Present at
                        BIO-Europe Spring Creative BioMart to Present at AACR Annual Meeting|Apr. 5-10, 2024|Booth #2953

Recombinant Horse IL12B protein, His & T7-tagged

Cat.No. : IL12B-846H
Product Overview : Recombinant Horse IL12B aa. (Ile23~Ser329 (Accession # Q9XSQ5)) fused with N-terminal His & T7 tag was produced in E. coli cells.
  • Specification
  • Gene Information
  • Related Products
Source : E. coli
Species : Horse
Tag : His & T7
Form : Freeze-dried powder
Molecular Mass : Predicted Molecular Mass: 38.6kDa
Protein length : Ile23~Ser329 (Accession # Q9XSQ5)
Endotoxin : <1.0EU per 1µg (determined by the LAL method)
Purity : > 95%
Characteristic : The isoelectric point is 5.8.
Applications : SDS-PAGE; WB; ELISA; IP
Stability : The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Storage : Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
Storage buffer : Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl
Reconstitution : Reconstitute in sterile PBS, pH7.2-pH7.4
Gene Name : IL12B interleukin 12B [ Equus caballus (horse) ]
Official Symbol : IL12B
Synonyms : IL12B; interleukin 12B; interleukin-12 subunit beta; CLMF p40; IL-12 p40; IL-12 subunit p40; IL-12B; cytotoxic lymphocyte maturation factor 40 kDa subunit; interleukin 12 p40 subunit; interleukin 12 subunit p40; interleukin 12B (natural killer cell stimulatory factor 2, cytotoxic lymphocyte maturation factor 2, p40)
Gene ID : 100034222
mRNA Refseq : NM_001082516.1
Protein Refseq : NP_001075985.1
UniProt ID : Q9XSQ5

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

Inquiry

  • Q&As
  • Reviews

Q&As (10)

Ask a question
Could you explain the use of ChIP-seq in studying IL12B transcriptional regulation? 05/12/2021

ChIP-seq identifies regulatory elements controlling IL12B gene expression.

Can you elaborate on the use of reporter assays to monitor IL12B promoter activity? 05/02/2021

Reporter assays monitor IL12B promoter activity under varying conditions.

What methods evaluate IL12B's response to different pathogenic stimuli in primary cells? 10/30/2020

Primary cell stimulation assays gauge IL12B response to pathogens.

How does siRNA knockdown of IL12B elucidate its role in immune cell activation? 05/25/2020

siRNA knockdown uncovers IL12B's role in immune cell activation pathways.

How does recombinant protein expression and purification aid in IL12B functional studies? 04/30/2020

Recombinant protein expression enables functional IL12B studies in vitro.

How does co-immunoprecipitation validate IL12B's association with signaling partners? 04/12/2018

Co-IP validates IL12B's binding to specific signaling partners.

What techniques are employed to analyze the impact of IL12B genetic variants on protein function? 12/03/2017

Genetic variant analysis reveals impacts on IL12B protein function.

How does flow cytometry reveal IL12B's expression dynamics in different immune cell subsets? 10/31/2017

Flow cytometry profiles IL12B expression across diverse immune cell types.

What experimental approaches determine IL12B's cytokine secretion kinetics upon stimulation? 09/29/2017

Cytokine secretion assays measure IL12B's response kinetics to stimuli.

What are the methods to assess IL12B's protein-protein interactions in a cellular context? 08/22/2017

Co-IP and immunoprecipitation assess IL12B's interactions with other proteins.

Customer Reviews (3)

Write a review
Reviews
04/08/2020

    Good for cytokine assays.

    01/06/2020

      Effective in Th2 cell differentiation.

      01/12/2019

        High yield in expression.

        Ask a Question for All IL12B Products

        Required fields are marked with *

        My Review for All IL12B Products

        Required fields are marked with *

        0

        Inquiry Basket

        cartIcon
        logo

        FOLLOW US

        Terms and Conditions        Privacy Policy

        Copyright © 2024 Creative BioMart. All Rights Reserved.

        Contact Us

        • /

        Stay Updated on the Latest Bioscience Trends