Recombinant Human ACADL protein, His-tagged

• Cat.No.: ACADL-1279H
• Product Overview: Recombinant Human ACADL(33 - 430 aa) fused with His tag was expressed in E. coli.

Specification

• Description: The protein encoded by this gene belongs to the acyl-CoA dehydrogenase family, which is a family of mitochondrial flavoenzymes involved in fatty acid and branched chain amino-acid metabolism. This protein is one of the four enzymes that catalyze the initial step of mitochondrial beta-oxidation of straight-chain fatty acid. Defects in this gene are the cause of long-chain acyl-CoA dehydrogenase (LCAD) deficiency, leading to nonketotic hypoglycemia.
• Source: E. coli
• Species: Human
• Tag: His
• Form: The purified protein was Lyophilized from sterile PBS (58mM Na2HPO4,17mM NaH2PO4, 68mM NaCl, pH7.). Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization.
• Protein length: 33 - 430 aa
• AA Sequence: EERLETPSAKKLTDIGIRRIFSPEH DIFRKSVRKFFQEEVIPHHSEWEKA GEVSREVWEKAGKQGLLGVNIAEHL GGIGGDLYSAAIVWEEQAYSNCSGP GFSIHSGIVMSYITNHGSEEQIKHF IPQMTAGKCIGAIAMTEPGAGSDLQ GIKTNAKKDGSDWILNGSKVFISNG SLSDVVIVVAVTNHEAPSPAHGISL FLVENGMKGFIKGRKLHKMGLKAQD TAELFFEDIRLPASALLGEENKGFY YIMKELPQERLLIADVAISASEFMF EETRNYVKQRKAFGKTVAHLQTVQH KLAELKTHICVTRAFVDNCLQLHEA KRLDSATACMAKYWASELQNSVAYD CVQLHGGWGYMWEYPIAKAYVDARV QPIYGGTNEIMKELIAREIVFDK
• Purity: 95%, by SDS-PAGE with Coomassie Brilliant Blue staining.
• Storage: Stability and Storage:
  Store for up to 12 months at -20 centigrade to -80 centigrade as lyophilized powder.
  Storage of Reconstituted Protein:
  Short-term storage: Store at 2-8 centigrade for two weeks.
  Long-term storage: Aliquot and store at -20 centigrade to -80 centigrade for up to 6 months, buffer containing 50% glycerol is recommended for reconstitution. Avoid repeat freeze-thaw cycles.
• Reconstitution: Reconstitute at 0.25 µg/μl in 200 μl sterile water for short-term storage.
  Reconstitution with 200 μl 50% glycerol solution is recommended for longer term storage (see Stability and Storage for more details).
  If a different concentration is needed for your purposes please adjust the reconstitution volume as required (please note: the ion concentration of the final solution will vary according to the volume used).
  Note: Centrifuge vial before opening. When reconstituting, gently pipet and wash down the sides of the vial to ensure full recovery of the protein into solution.
• Shipping: The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature (see below).

Gene Information

• Gene Name: ACADL acyl-CoA dehydrogenase, long chain [ Homo sapiens ]
• Official Symbol: ACADL
• Synonyms: ACADL; acyl-CoA dehydrogenase, long chain; acyl Coenzyme A dehydrogenase, long chain; long-chain specific acyl-CoA dehydrogenase, mitochondrial; ACAD4; LCAD; acyl-Coenzyme A dehydrogenase, long chain; FLJ94052
• Gene ID: 33
• mRNA Refseq: NM_001608
• Protein Refseq: NP_001599
• MIM: 609576
• UniProt ID: P28330
• Chromosome Location: 2q34-q35
• Pathway: Beta oxidation of lauroyl-CoA to decanoyl-CoA-CoA, organism-specific biosystem; Beta oxidation of myristoyl-CoA to lauroyl-CoA, organism-specific biosystem; Fatty Acid Beta Oxidation, organism-specific biosystem; Fatty acid metabolism, organism-specific biosystem; Fatty acid metabolism, conserved biosystem; Fatty acid, triacylglycerol, and ketone body metabolism, organism-specific biosystem; Metabolic pathways, organism-specific biosystem
• Function: acyl-CoA dehydrogenase activity; fatty-acyl-CoA binding; flavin adenine dinucleotide binding; long-chain-acyl-CoA dehydrogenase activity; palmitoyl-CoA oxidase activity