Recombinant Human ADD2 Protein, GST-tagged

• Cat.No.: ADD2-337H
• Product Overview: Human ADD2 full-length ORF ( AAH65525.1, 1 a.a. - 726 a.a.) recombinant protein with GST-tag at N-terminal.

Specification

• Description: Adducins are heteromeric proteins composed of different subunits referred to as adducin alpha, beta and gamma. The three subunits are encoded by distinct genes and belong to a family of membrane skeletal proteins involved in the assembly of spectrin-actin network in erythrocytes and at sites of cell-cell contact in epithelial tissues. While adducins alpha and gamma are ubiquitously expressed, the expression of adducin beta is restricted to brain and hematopoietic tissues. Adducin, originally purified from human erythrocytes, was found to be a heterodimer of adducins alpha and beta. Polymorphisms resulting in amino acid substitutions in these two subunits have been associated with the regulation of blood pressure in an animal model of hypertension. Heterodimers consisting of alpha and gamma subunits have also been described. Structurally, each subunit is comprised of two distinct domains. The amino-terminal region is protease resistant and globular in shape, while the carboxy-terminal region is protease sensitive. The latter contains multiple phosphorylation sites for protein kinase C, the binding site for calmodulin, and is required for association with spectrin and actin. Alternatively spliced transcript variants have been described. [provided by RefSeq, Jun 2010]
• Source: Wheat Germ
• Species: Human
• Tag: GST
• Molecular Mass: 107.2 kDa
• AA Sequence: MSEETVPEAASPPPPQGQPYFDRFS EDDPEYMRLRNRAADLRQDFNLMEQ KKRVTMILQSPSFREELEGLIQEQM KKGNNSSNIWALRQIADFMASTSHA VFPTSSMNVSMMTPINDLHTADSLN LAKGERLMRCKISSVYRLLDLYGWA QLSDTYVTLRVSKEQDHFLISPKGV SCSEVTASSLIKVNILGEVVEKGSS CFPVDTTGFCLHSAIYAARPDVRCI IHLHTPATAAVSAMKWGLLPVSHNA LLVGDMAYYDFNGEMEQEADRINLQ KCLGPTCKILVLRNHGVVALGDTVE EAFYKIFHLQAACEIQVSALSSAGG VENLILLEQEKHRPHEVGSVQWAGS TFGPMQKSRLGEHEFEALMRMLDNL GYRTGYTYRHPFVQEKTKHKSEVEI PATVTAFVFEEDGAPVPALRQHAQK QQKEKTRWLNTPNAYLRVNVADEVQ RSMGSPRPKTTWMKADEVEKSSSGM PIRIENPNQFVPLYTDPQEVLEMRN KIREQNRQDVKSAGPQSQLLASVIA EKSRSPSTESQLMSKGDEDTKDDSE ETVPNPFSQLTDQELEEYKKEVERK KLELDGEKETAPEEPGSPAKSAPAS PVQSPAKEAETKSPLVSPSKSLEEG TKKTETSKAATTEPETTQPEGVVVN GREEEQTAEEILSKGLSQMTTSADT DVDTSKDKTESVTSGPMSPEGSPSK SPSKKKKKFRTPSFLKKSKKKEKVE S
• Applications: Enzyme-linked Immunoabsorbent Assay
  Western Blot (Recombinant protein)
  Antibody Production
  Protein Array
• Notes: Best use within three months from the date of receipt of this protein.
• Storage: Store at -80 centigrade. Aliquot to avoid repeated freezing and thawing.
• Storage Buffer: 50 mM Tris-HCI, 10 mM reduced Glutathione, pH=8.0 in the elution buffer.

Gene Information

• Gene Name: ADD2 adducin 2 (beta) [ Homo sapiens ]
• Official Symbol: ADD2
• Synonyms: ADD2; adducin 2 (beta); beta-adducin; ADDB; erythrocyte adducin subunit beta
• Gene ID: 119
• mRNA Refseq: NM_001185054
• Protein Refseq: NP_001171983
• MIM: 102681
• UniProt ID: P35612