Recombinant Human AFP protein, M2 Peptide fused
Cat.No. : | AFP-29H |
Product Overview : | Recombinant Human AFP fusion protein was constructed by fusion human alpha fetal protein (AFP) N-terminal domain-I (1-198 aa) with human collagen type I derived AAQIRSQVMTHLRVIYER peptide and was expressed in E. coli. |
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Description : | Cell adhesion to the extracellular matrix (ECM) is essential for the development and maintenance of cells. Cell adhesion to various ECM as ligands is mainly mediated by cell surface receptor such as integrins. Human Collagen Type I is a key component in ECM for cell attachment and differentiation. M2 Peptide AAQIRSQVMTHLRVIYER that derived from human collagen type I protein has been demonstrated in vitro as collagen mimetics and specific interacts with APP. This peptide will be a good coating matrix for cell attachment in serum free medium or enhance specific cell lineage differentiation. |
Source : | E. coli |
Species : | Human |
Form : | 1.0 mg/ml, sterile-filtered, in 20 mM pH 8.0 Tris-HCl Buffer, with proprietary formulation of NaCl, KCl, EDTA, arginine, DTT and Glycerol. |
Purity : | >90% by SDS-PAGE |
Applications : | 1. As fusion protein fully derived from human protein, can be used as coating matrix protein to replace human collagen type I for human cell differentiation regulation study in vitro.2. Can be used in low serum or serum-free cell culture media to improve attachment and spreading of many normal and stem cells including human ES cell, endothelial, epithelial, hepatocytes and mesenchymal stem cells.3. Can be used to improve survival of primary cultures. |
Storage : | Keep at -80centigrade for long term storage. Product is stable at 4 centigrade for at least 30 days. |
Gene Name : | AFP alpha-fetoprotein [ Homo sapiens ] |
Official Symbol : | AFP |
Synonyms : | AFP; alpha-fetoprotein; HPAFP; FETA; alpha-fetoglobulin; alpha-1-fetoprotein; |
Gene ID : | 174 |
mRNA Refseq : | NM_001134 |
Protein Refseq : | NP_001125 |
MIM : | |
UniProt ID : | P02771 |
Chromosome Location : | 4q11-q13 |
Pathway : | Direct p53 effectors, organism-specific biosystem; FOXA2 and FOXA3 transcription factor networks, organism-specific biosystem; Glucocorticoid receptor regulatory network, organism-specific biosystem; |
Function : | metal ion binding; |
Products Types
◆ Recombinant Protein | ||
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AFP-082H | Recombinant Human AFP Protein, MYC/DDK-tagged, C13 and N15-labeled | +Inquiry |
AFP-1059H | Recombinant Human AFP Protein (Met1-Val609), HlgG1 Fc-tagged | +Inquiry |
◆ Native Protein | ||
AFP-3017H | Native Human fetal cord serum | +Inquiry |
AFP-3018P | Native pig AFP | +Inquiry |
AFP-8027H | Native Human Alpha FetoProtein | +Inquiry |
◆ Lysates | ||
AFP-1706HCL | Recombinant Human AFP cell lysate | +Inquiry |
Related Gene
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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Q&As (10)
Ask a questionThe clinical utility of AFP protein as a diagnostic or prognostic marker can be validated through clinical studies using patient samples, correlation analyses with disease outcomes, or comparison with existing diagnostic methods.
Downstream signaling pathways or cellular responses triggered by AFP protein binding or activation can be studied using techniques such as Western blotting, gene expression analysis, or functional assays specific to the signaling pathways of interest.
Therapeutic interventions or treatment strategies targeting AFP protein can be evaluated for efficacy and safety using in vitro or in vivo models, disease-specific assays, or clinical trials to assess their impact on disease progression, patient outcomes, or potential side effects.
The cellular and molecular mechanisms underlying the production and secretion of AFP protein can be elucidated through techniques such as gene expression analysis, protein trafficking studies, or immunofluorescence microscopy.
Genetic variations or mutations in the AFP gene that impact its expression or function can be investigated through genetic association studies, functional characterization of mutant variants, or population-based analyses.
The regulatory factors or signaling pathways that control the expression and transcriptional regulation of the AFP gene can be studied using techniques such as promoter analysis, transcription factor binding assays, or gene knockout/knockdown approaches.
The functional roles of AFP protein in embryonic development, tissue regeneration, or disease progression can be investigated through genetic or pharmacological perturbations, in vitro or in vivo model systems, or functional assays specific to the biological processes of interest.
Specific biomarkers or imaging techniques for detecting AFP protein in biological samples can be developed and their sensitivity and specificity can be assessed using techniques such as ELISA, immunohistochemistry, or imaging modalities like MRI or PET.
The interactions of AFP protein with its binding partners or ligands can be studied using techniques such as co-immunoprecipitation, surface plasmon resonance, or receptor-binding assays.
The immune-related functions of AFP protein, such as immunomodulation or immune evasion, can be investigated using techniques like immune cell assays, cytokine profiling, or immune response assays.
Customer Reviews (3)
Write a reviewInvestigating protein-protein interactions in cellular stress responses for cell survival.
Dissecting protein-protein interactions in viral replication for antiviral strategies.
Mapping protein-protein interactions in protein degradation pathways for proteostasis.
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