Recombinant Human ALOX5 Protein, GST-tagged

• Cat.No.: ALOX5-487H
• Product Overview: Human ALOX5 full-length ORF ( NP_000689.1, 1 a.a. - 674 a.a.) recombinant protein with GST-tag at N-terminal.

Specification

• Description: This gene encodes a member of the lipoxygenase gene family and plays a dual role in the synthesis of leukotrienes from arachidonic acid. The encoded protein, which is expressed specifically in bone marrow-derived cells, catalyzes the conversion of arachidonic acid to 5(S)-hydroperoxy-6-trans-8,11,14-cis-eicosatetraenoic acid, and further to the allylic epoxide 5(S)-trans-7,9-trans-11,14-cis-eicosatetrenoic acid (leukotriene A4). Leukotrienes are important mediators of a number of inflammatory and allergic conditions. Mutations in the promoter region of this gene lead to a diminished response to antileukotriene drugs used in the treatment of asthma and may also be associated with atherosclerosis and several cancers. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2012]
• Source: Wheat Germ
• Species: Human
• Tag: GST
• Molecular Mass: 104.4 kDa
• AA Sequence: MPSYTVTVATGSQWFAGTDDYIYLS LVGSAGCSEKHLLDKPFYNDFERGA VDSYDVTVDEELGEIQLVRIEKRKY WLNDDWYLKYITLKTPHGDYIEFPC YRWITGDVEVVLRDGRAKLARDDQI HILKQHRRKELETRQKQYRWMEWNP GFPLSIDAKCHKDLPRDIQFDSEKG VDFVLNYSKAMENLFINRFMHMFQS SWNDFADFEKIFVKISNTISERVMN HWQEDLMFGYQFLNGCNPVLIRRCT ELPEKLPVTTEMVECSLERQLSLEQ EVQQGNIFIVDFELLDGIDANKTDP CTLQFLAAPICLLYKNLANKIVPIA IQLNQIPGDENPIFLPSDAKYDWLL AKIWVRSSDFHVHQTITHLLRTHLV SEVFGIAMYRQLPAVHPIFKLLVAH VRFTIAINTKAREQLICECGLFDKA NATGGGGHVQMVQRAMKDLTYASLC FPEAIKARGMESKEDIPYYFYRDDG LLVWEAIRTFTAEVVDIYYEGDQVV EEDPELQDFVNDVYVYGMRGRKSSG FPKSVKSREQLSEYLTVVIFTASAQ HAAVNFGQYDWCSWIPNAPPTMRAP PPTAKGVVTIEQIVDTLPDRGRSCW HLGAVWALSQFQENELFLGMYPEEH FIEKPVKEAMARFRKNLEAIVSVIA ERNKKKQLPYYYLSPDRIPNSVAI
• Applications: Enzyme-linked Immunoabsorbent Assay; Western Blot (Recombinant protein); Antibody Production; Protein Array
• Notes: Best use within three months from the date of receipt of this protein.
• Storage: Store at -80 centigrade. Aliquot to avoid repeated freezing and thawing.
• Storage Buffer: 50 mM Tris-HCI, 10 mM reduced Glutathione, pH=8.0 in the elution buffer.

Gene Information

• Gene Name: ALOX5 arachidonate 5-lipoxygenase [ Homo sapiens ]
• Official Symbol: ALOX5
• Synonyms: ALOX5; arachidonate 5-lipoxygenase; 5 LOX; leukotriene A4 synthase; arachidonic acid 5-lipoxygenase; arachidonic 5-lipoxygenase alpha-10 isoform; arachidonic 5-lipoxygenase delta-13 isoform; arachidonic 5-lipoxygenase delta-p10 isoform; arachidonic 5-lipoxygenase delta-10-13 isoform; 5-LO; 5LPG; LOG5; 5-LOX; MGC163204
• Gene ID: 240
• mRNA Refseq: NM_000698
• Protein Refseq: NP_000689
• MIM: 152390
• UniProt ID: P09917