Recombinant Human AMPH Protein, His-tagged

• Cat.No.: AMPH-402H
• Product Overview: Recombinant Human AMPH(Val24~Lys241) fused with His tag at N-terminal was expressed in E. coli.

Specification

• Description: This gene encodes a protein associated with the cytoplasmic surface of synaptic vesicles. A subset of patients with stiff-man syndrome who were also affected by breast cancer are positive for autoantibodies against this protein. Alternate splicing of this gene results in two transcript variants encoding different isoforms. Additional splice variants have been described, but their full length sequences have not been determined. A pseudogene of this gene is found on chromosome 11.
• Source: E. coli
• Species: Human
• Tag: His
• Form: 100mMNaHCO3, 500mMNaCl, pH8.3, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.
• Molecular Mass: 29.3kDa
• Protein length: Val24~Lys241
• Endotoxin: <1.0EU per 1ug (determined by the LAL method)
• Purity: > 95%
• Applications: Positive Control; Immunogen; SDS-PAGE; WB.
  If bio-activity of the protein is needed, please check active protein
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 centigrade for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 centigrade for one month. Aliquot and store at -80 centigrade for 12 months.
• Reconstitution: Reconstitute in 100mM NaHCO3, 500mM NaCl (pH8.3) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Gene Information

• Gene Name: AMPH amphiphysin [ Homo sapiens ]
• Official Symbol: AMPH
• Synonyms: AMPH; amphiphysin; amphiphysin (Stiff Man syndrome with breast cancer 128kDa autoantigen) , amphiphysin (Stiff Mann syndrome with breast cancer 128kD autoantigen); amphiphysin I; amphiphysin (Stiff-Mann syndrome with breast cancer 128kD autoantigen); AMPH1
• Gene ID: 273
• mRNA Refseq: NM_001635
• Protein Refseq: NP_001626
• MIM: 600418
• UniProt ID: P49418