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Recombinant Human API5 protein, His-tagged

Cat.No. : API5-1171H
Product Overview : Recombinant Human API5 aa. (Pro2~Phe360 fused with N-terminal His tag was produced in E. coli cells.
  • Specification
  • Gene Information
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Description : This gene encodes an apoptosis inhibitory protein whose expression prevents apoptosis after growth factor deprivation. This protein suppresses the transcription factor E2F1-induced apoptosis and also interacts with, and negatively regulates Acinus, a nuclear factor involved in apoptotic DNA fragmentation. Its depletion enhances the cytotoxic action of the chemotherapeutic drugs. Multiple alternatively spliced transcript variants encoding different isoforms have been identified.
Source : E. coli
Species : Human
Tag : His
Form : Freeze-dried powder
Molecular Mass : 44kDa as determined by SDS-PAGE reducing conditions.
Protein length : Pro2~Phe360
Endotoxin : <1.0EU per 1µg (determined by the LAL method)
Purity : >97%
Characteristic : The isoelectric point is 4.7.
Applications : Positive Control; Immunogen; SDS-PAGE; WB.
Stability : The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Storage : Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
Concentration : 200μg/mL
Storage buffer : 20mM Tris, 150mM NaCl, pH8.0, containing 0.01% SKL, 5% Trehalose and Proclin300.
Reconstitution : Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
Gene Name : API5 apoptosis inhibitor 5 [ Homo sapiens (human) ]
Official Symbol : API5
Synonyms : API5; apoptosis inhibitor 5; AAC11; AAC-11; FIF; antiapoptosis clone 11 protein; cell migration-inducing gene 8 protein; fibroblast growth factor 2-interacting factor 2; migration-inducing protein MIG8
Gene ID : 8539
mRNA Refseq : NM_001142930.1
Protein Refseq : NP_001136402.1
UniProt ID : Q9BZZ5

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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Q&As (20)

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What are the detection methods for API5 gene mutation in tumor patients? 09/17/2022

The mutation of API5 gene can be detected by PCR, sequencing, immunohistochemistry and other methods.

What is the application prospect of API5 in prognostic diagnosis? 09/11/2022

Recombinant API5 protein has an important clinical application prospect in prognostic diagnosis, and can provide an important basis for accurate treatment.

What is the role of API5 in the process of tumor metastasis? 06/26/2022

Recombinant API5 protein can promote the metastasis of tumor cells and further promote tumor growth and progression.

What are the adverse reactions of API5 in antitumor therapy? 08/30/2021

The specific adverse reactions need to be determined on a case-by-case basis. So far, studies have shown no serious adverse reactions.

How to evaluate the efficacy of API5 in tumor therapy? 07/12/2021

Clinical trials can be conducted to evaluate the efficacy of recombinant API5 protein in the treatment of tumors.

What is the prospect of recombinant API5 protein in the treatment of tumors? 06/08/2021

Current studies have shown that recombinant API5 protein can play important biological functions in cancer cells and their microenvironment, and has a wide range of therapeutic prospects.

How to improve the targeting and specificity of API5 ? 05/07/2021

The targeting and specificity of API5 can be improved by constructing targeted drugs, probes, antibodies and other means.

What is the role of API5 in tumor radiotherapy? 03/13/2021

Recombinant API5 protein can enhance the tolerance of tumor cells to radiotherapy by inhibiting apoptosis of tumor cells.

What is the main function of API5 ? 03/11/2021

API5 is an inhibitor of apoptosis, which has the function of inhibiting apoptosis in cells and promoting cell survival.

What are the interactions between API5 s and other proteins? 10/31/2020

Recombinant API5 proteins can bind to other apoptosis-related cytokines and proteins, thereby regulating cell survival and apoptosis signaling pathways.

What are the potential advantages of the combination of API5 and tumor immunotherapy? 07/08/2020

Recombinant API5 protein can enhance the effect of tumor immunotherapy by inhibiting the apoptosis of immune cells.

How to optimize the expression and purification of API5 ? 07/01/2020

The expression and purification of API5 can be optimized by optimizing the carrier, culture conditions, purification methods and other means.

How to improve the stability of API5 in vivo? 06/27/2020

The stability of API5 in vivo can be improved by building a stable expression system, nucleic acid drug targeting, nanotechnology and other means.

What is the genetic structure of API5 ? 06/11/2020

The protein encoded by the API5 gene is composed of 359 amino acids with a molecular weight of about 40kDa and contains a apoptosis inhibition domain (AID) domain.

How to detect the expression level of API5 ? 04/12/2020

The expression level of API5 can be detected by Western blot, immunohistochemistry, PCR and other methods.

How to efficiently deliver API5 to tumor cells? 01/26/2020

The recombinant API5 protein can be efficiently delivered to tumor cells by means of nanotechnology, gene transfection, cell delivery, etc.

How to evaluate the pharmacokinetics and toxicology of API5 ? 11/27/2019

The pharmacokinetics and toxicology of API5 s can be evaluated through animal studies and in vitro studies.

How to improve the biological activity of API5 ? 10/22/2019

The biological activity of API5 can be improved by point mutation, structural optimization and sensitivity improvement.

In which diseases does recombinant API5 play an important role? 07/07/2019

Recombinant API5 protein plays an important role in the occurrence, growth, metastasis and progression of a variety of tumors.

How to prepare API5 ? 04/06/2019

Recombinant API5 proteins can usually be prepared in E. coli or eukaryotic cells by gene recombination techniques.

Customer Reviews (3)

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Reviews
09/17/2022

    Characteristics of short half-life and high clearance provide greater potential for recombinant proteins in the treatment of various diseases, and are expected to become a new generation of therapeutic drugs.

    08/06/2020

      The stability of API5 is excellent, and its structure and function remain stable even after multiple freeze-thaw cycles, showing excellent product quality.

      05/16/2019

        Western blot experiments using API5 can obtain very consistent results, indicating its high repeatability and reproducibility.

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