Recombinant Human APOL1 Protein, His-tagged

• Cat.No.: APOL1-582H
• Product Overview: Recombinant human APOL1 (Met 1-Leu398) (Q2KHQ6) was expressed, with a C-terminal polyhistidine tag.

Specification

• Source: Baculovirus-Insect Cells
• Species: Human
• Tag: His
• Predicted N Terminal: Glu 28
• Form: Lyophilized from sterile 20mM Tris, 500mM NaCl, pH 7.4, 10% gly, 3mM DTT, 5%~8% trehalose and mannitol.
• Molecular Mass: The secreted recombinant human APOL1 consists of 381 amino acids and predicts a molecular mass of 42.5 KDa. The apparent molecular mass of the protein is approximately 44 KDa in SDS-PAGE under reducing conditions due to glycosylation.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
• Purity: >91 % as determined by SDS-PAGE.
• Stability: Samples are stable for up to twelve months from date of receipt at -70ºC.
• Storage: Store it under sterile conditions at -20ºC~-70ºC. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.25 ug/ul. Centrifuge the vial at 4℃ before opening to recover the entire contents.

Gene Information

• Gene Name: APOL1 apolipoprotein L, 1 [ Homo sapiens ]
• Official Symbol: APOL1
• Synonyms: APOL1; apolipoprotein L, 1; APOL; apolipoprotein L1; APO-L; FSGS4; APOL-I
• Gene ID: 8542
• mRNA Refseq: NM_001136540
• Protein Refseq: NP_001130012
• MIM: 603743
• UniProt ID: O14791
• Chromosome Location: 22q13.1
• Pathway: African trypanosomiasis, organism-specific biosystem; African trypanosomiasis, conserved biosystem