"BTLA" Related Products

Recombinant Human BTLA, Fc-tagged

Cat.No.: BTLA-192H
Product Overview: Human BTLA (31-134) Protein, Fc Tag (Human BTLA (31-134), Fc Tag) Lys 31 - Thr 134 (Accession # NP_001078826.1) was produced in human 293 cells (HEK293).
Source: HEK293
Species: Human
Tag: Fc
Form: Lyophilized from 0.22 μm filtered solution in 50 mM tris, 100 mM glycine, pH7.5, 10% Trehalose.
Bio-activity: Measured by its binding ability in a functional ELISA.Immobilized Human HVEM, Strep Tag at 2 μg/mL (100 μL/well) can bind Human BTLA, Fc Tag with a linear range of 0.01 – 0.5 μg/mL.
Molecular Mass: Human BTLA (31-134), Fc Tag is fused with a human IgG1 Fc tag at the C-terminus, and has a calculated MW of 38.7 kDa. The predicted N-terminus is Lys 31. The reducing (R) protein migrates as 45-50 kDa in SDS-PAGE due to glycosylation.
Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
Purity: >92% as determined by SDS-PAGE.
Stability: Samples are stable for up to twelve months from date of receipt at -70 centigrade
Storage: Store it under sterile conditions at -20 centigrade to -80 centigrade. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4ºC before opening to recover the entire contents.
Gene Name: BTLA B and T lymphocyte associated [ Homo sapiens ]
Official Symbol: BTLA
Synonyms: BTLA; B and T lymphocyte associated; B- and T-lymphocyte attenuator; BTLA1; CD272; B and T lymphocyte attenuator; B- and T-lymphocyte-associated protein; FLJ16065; MGC129743;
Gene ID: 151888
mRNA Refseq: NM_001085357
Protein Refseq: NP_001078826
MIM: 607925
UniProt ID: Q7Z6A9
Chromosome Location: 3q13.2
Pathway: Adaptive Immune System, organism-specific biosystem; Costimulation by the CD28 family, organism-specific biosystem; Immune System, organism-specific biosystem;
Function: receptor activity;

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Optional requirements on this protein    +Expand
C-fusion    N-fusion   Non-tagged
His    GST   Fc   Others
<1.0 eu/μg    <0.1 eu/μg   <0.01 eu/μg   Not required
Monomer Isolation    Dimer Isolation    Not required
>80% by SDS-PAGE    >90% by SDS-PAGE   >95% by SDS-PAGE   Others

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