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Home > Products > Recombinant Proteins > Recombinant Human CD226 protein, His-tagged

Recombinant Human CD226 protein, His-tagged

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Cat.No.:
CD226-229H
Product Overview:
Recombinant Human CD226(Glu19-Asn247) fused with His tag at C-terminal was expressed in HEK293.
Description:
DNAX accessory molecule-1 (DNAM-1), also known as CD226, is a 65 kDa type I transmembrane glycoprotein in the immunoglobulin superfamily (1). Mature human DNAM-1 contains a 236 amino acid (aa) extracellular domain (ECD) with two Ig-like C2-set domains and a 61 aa cytoplasmic region that contains motifs for binding PDZ domains and band 4.1 family proteins. Within the ECD, human DNAM-1 shares 50% and 52% aa sequence identity with mouse and rat DNAM-1, respectively. DNAM-1 is expressed on multiple lymphoid and myeloid cells and interacts with CD155/PVR and Nectin-2/CD112. Ligation of DNAM-1 promotes the activation of NK cells, CD8+ T cells, and mast cells, dendritic cell maturation, megakaryocyte and activated platelet adhesion to vascular endothelial cells, and monocyte extravasation; it inhibits the formation of osteoclasts. Platelet-endothelium interactions mediated by DNAM-1 can enable the metastasis of tumor cells to the lung. CD96 competes with DNAM-1 for binding to CD155 and blocks DNAM-1 mediated NK cell activation. In activated, but not in resting NK, T, and mast cells, the cis association of DNAM-1 with CD18 contributes to the tyrosine and serine phosphorylation of DNAM-1 during activation.
Source:
HEK293
Species:
Human
Tag :
His
Predicted N Terminal:
Glu19
Form:
Lyophilized from a 0.2 μm filtered solution in PBS.
Bio-activity:
Measured by its binding ability in a functional ELISA. When Recombinant Human DNAM-1/CD226 is immobilized at 0.5 µg/mL (100 µL/well), Recombinant Human Nectin-2/CD112 Fc Chimera binds with an ED50 of 0.4-2 μg/mL.
Molecular Mass:
Predicted Molecular Mass: 27 kDa
SDS-PAGE: 31-55 kDa, reducing conditions
Endotoxin:
<0.1 EU per 1 μg of the protein by the LAL method.
Purity:
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Storage:
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 centigrade as supplied.
1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
Reconstitution:
Reconstitute at 250 μg/mL in PBS.


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Note: There will be extra charge for optional service!
Optional requirements on this protein +Expand
C-fusion   N-fusion  Non-tagged
His   GST  Fc  Others
<1.0 eu/μg   <0.1 eu/μg  <0.01 eu/μg  Not required
Monomer Isolation   Dimer Isolation   Not required
>80% by SDS-PAGE   >90% by SDS-PAGE  >95% by SDS-PAGE  Others
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45-1 Ramsey Road, Shirley, NY 11967, USA
USA: 1-631-559-9269  1-631-448-7888
Europe: 44-207-048-3343
FAX: 1-631-938-8127
EMAIL: info@creative-biomart.com

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