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Recombinant Human CD226 protein, His-tagged

Cat.No.: CD226-229H
Product Overview: Recombinant Human CD226(Glu19-Asn247) fused with His tag at C-terminal was expressed in HEK293.
Description: DNAX accessory molecule-1 (DNAM-1), also known as CD226, is a 65 kDa type I transmembrane glycoprotein in the immunoglobulin superfamily (1). Mature human DNAM-1 contains a 236 amino acid (aa) extracellular domain (ECD) with two Ig-like C2-set domains and a 61 aa cytoplasmic region that contains motifs for binding PDZ domains and band 4.1 family proteins. Within the ECD, human DNAM-1 shares 50% and 52% aa sequence identity with mouse and rat DNAM-1, respectively. DNAM-1 is expressed on multiple lymphoid and myeloid cells and interacts with CD155/PVR and Nectin-2/CD112. Ligation of DNAM-1 promotes the activation of NK cells, CD8+ T cells, and mast cells, dendritic cell maturation, megakaryocyte and activated platelet adhesion to vascular endothelial cells, and monocyte extravasation; it inhibits the formation of osteoclasts. Platelet-endothelium interactions mediated by DNAM-1 can enable the metastasis of tumor cells to the lung. CD96 competes with DNAM-1 for binding to CD155 and blocks DNAM-1 mediated NK cell activation. In activated, but not in resting NK, T, and mast cells, the cis association of DNAM-1 with CD18 contributes to the tyrosine and serine phosphorylation of DNAM-1 during activation.
Source: HEK293
Species: Human
Tag : His
Predicted N Terminal: Glu19
Form: Lyophilized from a 0.2 μm filtered solution in PBS.
Bio-activity: Measured by its binding ability in a functional ELISA. When Recombinant Human DNAM-1/CD226 is immobilized at 0.5 µg/mL (100 µL/well), Recombinant Human Nectin-2/CD112 Fc Chimera binds with an ED50 of 0.4-2 μg/mL.
Molecular Mass: Predicted Molecular Mass: 27 kDa
SDS-PAGE: 31-55 kDa, reducing conditions
Endotoxin: <0.1 EU per 1 μg of the protein by the LAL method.
Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 centigrade as supplied.
1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
Reconstitution: Reconstitute at 250 μg/mL in PBS.
Gene Name: CD226 CD226 molecule [ Homo sapiens ]
Official Symbol: CD226
Synonyms: CD226; CD226 molecule; CD226 antigen; DNAM 1; DNAM1; PTA1; TLiSA1; adhesion glycoprotein; DNAX accessory molecule 1; DNAX accessory molecule-1; platelet and T cell activation antigen 1; T lineage-specific activation antigen 1 antigen; DNAM-1;
Gene ID: 10666
mRNA Refseq: NM_006566
Protein Refseq: NP_006557
MIM: 605397
UniProt ID: Q15762

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Optional requirements on this protein    +Expand
C-fusion    N-fusion   Non-tagged
His    GST   Fc   Others
<1.0 eu/μg    <0.1 eu/μg   <0.01 eu/μg   Not required
Monomer Isolation    Dimer Isolation    Not required
>80% by SDS-PAGE    >90% by SDS-PAGE   >95% by SDS-PAGE   Others

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