Address: 45-1 Ramsey Road, Shirley, NY 11967, USA    USA: 1-631-559-9269  1-631-448-7888   Fax: 1-631-938-8127  Europe: 44-207-048-3343   Email: info@creative-biomart.com
Home > Products > Recombinant Proteins > Recombinant Human CD244 protein, Fc & Avi-tagged, Biotinylated

Recombinant Human CD244 protein, Fc & Avi-tagged, Biotinylated

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Cat.No.:
CD244-1825H
Product Overview:
Biotinylated Human 2B4, Fc Tag, Avi Tag is expressed from human 293 cells (HEK293). It contains AA Cys 22 - Arg 221 (Accession # NP_057466.1). Biotinylation of this product is performed using Avitag technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin. The biotin to protein ratio is 0.5-1 as determined by the HABA assay.
Description:
Natural killer cell receptor 2B4 is also known as NK cell type I receptor protein 2B4 (NKR2B4 or h2B4), SLAM family member 4 (SLAMF4), Signaling lymphocytic activation molecule 4, CD antigen CD244. NKR2B4 / CD244 contains two Ig-like (immunoglobulin-like) domains. CD244 is expressed in spleen, PBL, followed by lung, liver, testis and small intestine. CD244 interacts with CD48. Following phosphorylation, CD244 is able to recruit PTPN11/SHP-2 and SH2D1A/SAP. SLAMF4 modulate other receptor-ligand interactions to enhance leukocyte activation. CD244/2B4 is the only heterophilic receptor of SLAM family.
Source:
HEK293
Species:
Human
Tag :
Fc & Avi
Predicted N Terminal:
Cys 22
Form:
Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, pH 7.5, 10% trehalose.
Bio-activity:
Immobilized Human CD48, His Tag at 10 μg/mL (100 μL/well) can bind Biotinylated Human 2B4, Fc Tag with a linear range of 0.16-1.25 μg/mL.
Molecular Mass:
This protein carries a human IgG1 Fc tag at the C-terminus, followed by an Avi tag. The protein has a calculated MW of 50.7 kDa. As a result of glycosylation, the protein migrates as 65-80 kDa under reducing (R) condition, and 130-160 kDa under non-reducing (NR) condition (SDS-PAGE).
Protein length:
AA Cys 22 - Arg 221
Endotoxin:
Less than 1.0 EU per μg by the LAL method.
Purity:
>95% as determined by SDS-PAGE.
Storage:
For long term storage, the product should be stored at lyophilized state at -20°C or lower. Please avoid repeated freeze-thaw cycles. No activity loss is observed after storage at: 4-8°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution.
Reconstitution:
It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4ºC before opening to recover the entire contents.


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Optional requirements on this protein +Expand
C-fusion   N-fusion  Non-tagged
His   GST  Fc  Others
<1.0 eu/μg   <0.1 eu/μg  <0.01 eu/μg  Not required
Monomer Isolation   Dimer Isolation   Not required
>80% by SDS-PAGE   >90% by SDS-PAGE  >95% by SDS-PAGE  Others
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45-1 Ramsey Road, Shirley, NY 11967, USA
USA: 1-631-559-9269  1-631-448-7888
Europe: 44-207-048-3343
FAX: 1-631-938-8127
EMAIL: info@creative-biomart.com

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