Recombinant Human CD44 protein, GST-tagged

• Cat.No.: CD44-589H
• Product Overview: Recombinant Human CD44(28-380aa) fused with GST tag at N-terminal was expressed in E. coli.

Specification

• Description: The protein encoded by this gene is a cell-surface glycoprotein involved in cell-cell interactions, cell adhesion and migration. It is a receptor for hyaluronic acid (HA) and can also interact with other ligands, such as osteopontin, collagens, and matrix metalloproteinases (MMPs). This protein participates in a wide variety of cellular functions including lymphocyte activation, recirculation and homing, hematopoiesis, and tumor metastasis. Transcripts for this gene undergo complex alternative splicing that results in many functionally distinct isoforms, however, the full length nature of some of these variants has not been determined. Alternative splicing is the basis for the structural and functional diversity of this protein, and may be related to tumor metastasis.
• Source: E. coli
• Species: Human
• Tag: GST
• Form: 1M PBS (58 mM Na2HPO4, 17 mM NaH2PO4, 68 mM NaCl, pH8.0 ), 100 mM GSH and 1% Triton X-100, 15% glycerol.
• Protein length: 28-380aa
• AA Sequence: CRFAGVFHVEKNGRYSISRTEAADL CKAFNSTLPTMAQMEKALSIGFETC RYGFIEGHVVIPRIHPNSICAANNT GVYILTSNTSQYDTYCFNASAPPEE DCTSVTDLPNAFDGPITITIVNRDG TRYVQKGEYRTNPEDIYPSNPTDDD VSSGSSSERSSTSGGYIFYTFSTVH PIPDEDSPWITDSTDRIPATSTSSN TISAGWEPNEENEDERDRHLSFSGS GIDDDEDFISSTISTTPRAFDHTKQ NQDWTQWNPSHSNPEVLLQTTTRMT DVDRNGTTAYEGNWNPEAHPPLIHH EHHEEEETPHSTSTIQATPSSTTEE TATQKEQWFGNRWHEGYRQTPREDS HST
• Stability: Aliquot and store at -20 centigrade to -80 centigrade for up to 6 months. Avoid freeze thaw cycles.
• Storage: Short-term storage: Store at 2-8 centigrade for two weeks. Long-term storage: Aliquot and store at -20 centigrade to -80 centigrade for up to 6 months, buffer containing 50% glycerol is recommended for Reconstitution. Avoid repeat freeze-thaw cycles.
• Reconstitution: Reconstitute at 0.25 µg/μl in 200 μl sterile water for short-term storage. Reconstitution with 200 μl 50% glycerol solution is recommended for longer term storage (see Stability and Storage for more details). If a different concentration is needed for your purposes please adjust the reconstitution volume as required (please note: the ion concentration of the final solution will vary according to the volume used). Note: Centrifuge vial before opening. When Reconstituting, gently pipet and wash down the sides of the vial to ensure full recovery of the protein into solution.
• Shipping: The product is shipped with ice packs. Upon receipt, store it immediately at -20 centigrade to -80 centigrade.

Gene Information

• Gene Name: CD44 CD44 molecule (Indian blood group) [ Homo sapiens ]
• Official Symbol: CD44
• Synonyms: CD44; CD44 molecule (Indian blood group); CD44 antigen (homing function and Indian blood group system) , MDU2, MDU3, MIC4; CD44 antigen; CD44R; chondroitin sulfate proteoglycan 8; CSPG8; HCELL; hematopoietic cell E and L selectin ligand; IN; MC56; Pgp1; epican; Hermes antigen; hyaluronate receptor; phagocytic glycoprotein 1; heparan sulfate proteoglycan; cell surface glycoprotein CD44; extracellular matrix receptor III; GP90 lymphocyte homing/adhesion receptor; hematopoietic cell E- and L-selectin ligand; homing function and Indian blood group system; LHR; MDU2; MDU3; MIC4; CDW44; HUTCH-I; ECMR-III; MGC10468
• Gene ID: 960
• mRNA Refseq: NM_000610
• Protein Refseq: NP_000601
• MIM: 107269
• UniProt ID: P16070
• Chromosome Location: 11p13
• Pathway: Cytokine Signaling in Immune system, organism-specific biosystem; ECM-receptor interaction, organism-specific biosystem; ECM-receptor interaction, conserved biosystem; Hematopoietic cell lineage, organism-specific biosystem; Hematopoietic cell lineage, conserved biosystem; Immune System, organism-specific biosystem; Interferon Signaling, organism-specific biosystem
• Function: binding; collagen binding; hyaluronic acid binding; hyaluronic acid binding; hyaluronic acid binding; hyalurononglucosaminidase activity; protein binding; receptor activity; contributes_to transmembrane signaling receptor activity