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Home > Products > Recombinant Proteins > Recombinant Human CDK2/CCNE2 Active protein, GST-tagged

Recombinant Human CDK2/CCNE2 Active protein, GST-tagged

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"CDK2/CCNE2" Related Products.
By Species
Human(1)
By Source
By Tag
GST(1)
Cat.No.:
CDK2-CCNE2-311H
Product Overview:
Recombinant full-length human CDK2 and CCNE2 were co-expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag on both proteins.
Description:
CDK2 is a member of the Cyclin-Dependent Kinase family that is ubiquitously expressed. CDK2 is a catalytic subunit of the cyclin-dependent protein kinase complex, whose activity is restricted to the G1-S phase, and essential for cell cycle G1/S phase transition. CDK2 associates with and is regulated by the regulatory subunits of the complex including Cyclin A or E, CDK inhibitor p21Cip1 (CDKN1A) and p27Kip1 (CDKN1B). CDK2 phosphorylates multiple cellular substrates including SMAD3 and FOXO1. Phosphorylation of FOXO1 leads to its inhibition.
Source:
Insect cell
Species:
Human
Tag :
GST
Form:
50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol
Bio-activity:
The specific activity of CDK2/CyclinE2 was determined to be 60nmol/min/mg as per activity protocol.
Molecular Mass:
CDK2 ~58 kDa and CCNE2 ~72 kDa
Purity:
The purity of CDK2/CCNE2 was determined to be >90% by densitometry.
Applications:
Kinase Assay; WB
Stability:
1 year at -70 centigrade
Storage:
Store product at -70 centigrade. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Concentration:
0.1 μg/μL


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Optional requirements on this protein +Expand
C-fusion   N-fusion  Non-tagged
His   GST  Fc  Others
<1.0 eu/μg   <0.1 eu/μg  <0.01 eu/μg  Not required
Monomer Isolation   Dimer Isolation   Not required
>80% by SDS-PAGE   >90% by SDS-PAGE  >95% by SDS-PAGE  Others
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45-1 Ramsey Road, Shirley, NY 11967, USA
USA: 1-631-559-9269  1-631-448-7888
Europe: 44-207-097-1828
FAX: 1-631-938-8127
EMAIL: info@creative-biomart.com

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