Active Recombinant Human CDK3/CCNE2 protein, GST-tagged
Cat.No. : | CDK3-CCNE2-312H |
Product Overview : | Recombinant full-length human CDK3 and CCNE2 were co-expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag on both proteins. |
- Specification
- Gene Information
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Description : | CDK3/CyclinE is a member of the cyclin-dependent protein kinase family and promotes entry into S phase, in part by activating members of the E2F family of transcription factors. CDK3 can also associate with cyclin C and phosphorylates the retinoblastoma 1 protein to promote exit from G0. The CDK3 gene has been mapped to chromosomal location of 17q22-qter, telomeric to the BRCA1 gene by somatic cell hybrids analysis. The presence of a single point mutation in the CDK3 gene from several Mus musculus strains commonly used in the laboratory has been reported. |
Source : | Insect cell |
Species : | Human |
Tag : | GST |
Form : | 50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol |
Bio-activity : | The specific activity of CDK3/CyclinE2 was determined to be 80nmol/min/mg as per activity protocol. |
Molecular Mass : | CDK3 ~60 kDa and CCNE2 ~72 kDa |
Purity : | The purity of CDK3/CCNE2 was determined to be >95% by densitometry. |
Applications : | Kinase Assay; WB |
Stability : | 1 year at -70 centigrade |
Storage : | Store product at -70 centigrade. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles. |
Concentration : | 0.1 μg/μL |
Gene Name : | cyclin dependent kinase 3 [ Homo sapiens (human) ]; cyclin E2 [ Homo sapiens (human) ] |
Official Symbol : | CDK3; CCNE2 |
Synonyms : | CDK3; CCNE2; CYCE2 |
Gene ID : | 1018; 9134 |
mRNA Refseq : | NM_001258; NM_057749 |
Protein Refseq : | NP_001249; NP_001228 |
MIM : | 123828; 603775 |
UniProt ID : | Q00526; P24864 |
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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