Recombinant Human CDK8/CCNC protein, GST-tagged

• Cat.No.: CDK8-CCNC-314H
• Product Overview: Recombinant full-length human CDK8/CCNC was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.

Specification

• Description: CDK8 is a member of the CDK family which are important regulators of cell cycle progression. CDK8 associates with Cyclin C and is a component of the RNA polymerase II holoenzyme complex that phosphorylates the carboxy-terminal domain (CTD) of the largest subunit of RNA polymerase II. CDK8 regulate transcription by targeting the CDK7/cyclin H subunits of the general transcription initiation factor IIH (TFIIH). Inhibition of CDK8 expression inhibits proliferation in colon cancer cells characterized by high levels of CDK8 and beta-catenin hyperactivity. CDK8 kinase activity is necessary for β-catenin-driven transformation and for expression of several β-catenin transcriptional targets.
• Source: Insect cell
• Species: Human
• Tag: GST
• Form: 50mM Tris-HCl, pH 7.5, 50mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol
• Molecular Mass: CDK8 ~84kDa and CCNC ~55 kDa
• Purity: The purity of CDK8/CCNC was determined to be >70% by densitometry.
• Applications: WB
• Stability: 1 year at -70 centigrade
• Storage: Store product at -70 centigrade. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
• Concentration: 0.1 μg/μL

Gene Information

• Gene Name: cyclin dependent kinase 8 [ Homo sapiens (human) ]; cyclin C [ Homo sapiens (human) ]
• Official Symbol: CDK8; CCNC
• Synonyms: CDK8: K35, MGC126074, MGC126075; CyclinC; CCNC; CycC
• Gene ID: 1024; 892
• mRNA Refseq: NM_001260; NM_005190
• Protein Refseq: NP_001251; NP_005181.2
• MIM: 603184; 123838
• UniProt ID: P49336; P24863