Creative BioMart to Present at
                        BIO-Europe Spring Creative BioMart to Present at AACR Annual Meeting|Apr. 5-10, 2024|Booth #2953

Recombinant Human CHAT protein, His-tagged

Cat.No. : CHAT-172H
Product Overview : Recombinant Human CHAT(Asp517~Pro732) fused with His tag at N-terminal was expressed in E. coli.
Unit
Price
Qty
  • Specification
  • Gene Information
  • Related Products
Description : This gene encodes an enzyme which catalyzes the biosynthesis of the neurotransmitter acetylcholine. This gene product is a characteristic feature of cholinergic neurons, and changes in these neurons may explain some of the symptoms of Alzheimer's disease. Polymorphisms in this gene have been associated with Alzheimer's disease and mild cognitive impairment. Mutations in this gene are associated with congenital myasthenic syndrome associated with episodic apnea. Multiple transcript variants encoding different isoforms have been found for this gene, and some of these variants have been shown to encode more than one isoform.
Source : E. coli
Species : Human
Tag : His
Form : PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300
Molecular Mass : 28.0kDa
Protein length : Asp517~Pro732
Endotoxin : <1.0EU per 1µg (determined by the LAL method)
Purity : > 95%
Applications : Positive Control; Immunogen; SDS-PAGE; WB.
If bio-activity of the protein is needed, please check active protein
Stability : The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 centigrade for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Storage : Avoid repeated freeze/thaw cycles. Store at 2-8 centigrade for one month. Aliquot and store at -80 centigrade for 12 months.
Reconstitution : Reconstitute in PBS or others.
Gene Name : CHAT choline O-acetyltransferase [ Homo sapiens ]
Official Symbol : CHAT
Synonyms : CHAT; choline O-acetyltransferase; choline acetyltransferase; choline acetylase; acetyl CoA:choline O-acetyltransferase; CMS1A; CMS1A2; CHOACTASE;
Gene ID : 1103
mRNA Refseq : NM_001142929
Protein Refseq : NP_001136401
MIM : 118490
UniProt ID : P28329
Chromosome Location : 10q11.2
Pathway : Acetylcholine Neurotransmitter Release Cycle, organism-specific biosystem; Acetylcholine Synthesis, organism-specific biosystem; Biogenic Amine Synthesis, organism-specific biosystem; Cholinergic synapse, organism-specific biosystem; Glycerophospholipid metabolism, organism-specific biosystem; Glycerophospholipid metabolism, conserved biosystem; Neuronal System, organism-specific biosystem;
Function : choline O-acetyltransferase activity; choline binding; transferase activity, transferring acyl groups;

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

Inquiry

  • Q&As
  • Reviews

Q&As (6)

Ask a question
How to use the activity of CHAT protein for disease treatment? 10/14/2022

The activity of the CHAT protein can be modulated by drugs or other small molecule compounds to treat neurological disorders associated with CHAT protein abnormalities. For example, some drugs can promote the expression and activity of the CHAT protein, thereby increasing acetylcholine synthesis.

How is the expression level of CHAT protein regulated? 10/15/2021

Expression level of CHAT protein is regulated by a variety of factors, including gene expression, post-transcriptional modifications, nutritional status, etc. Some drugs or other small molecule compounds can also affect their expression levels by regulating gene expression or post-transcriptional modifications.

What diseases are mutations or abnormal expression of the CHAT protein associated with? 04/21/2021

Some studies have shown that mutations or abnormal expression of the CHAT protein may be related to neurological diseases, psychiatric diseases, etc. However, further research is needed to confirm the association.

How are CHAT proteins related to other neurotransmitters or signaling molecules? 11/24/2020

CHAT protein has some association with other neurotransmitters or signaling molecules. For example, it can interact with cholinergic neurons to co-regulate acetylcholine synthesis and release. In addition, it can interact with other neurotransmitters or signaling molecules such as dopamine, glutamate, etc.

How to use CHAT protein for disease diagnosis? 03/09/2020

The occurrence, progression and prognosis of neurological diseases can be assessed by detecting characteristics such as the expression level, activity and interaction of CHAT protein with other proteins. These features can also be used to develop new biomarkers and drug targets.

Why are CHAT proteins linked to disease development and progression? 06/15/2019

Some studies have shown that abnormal expression of CHAT protein may be related to the occurrence and progression of some neurological diseases, such as Alzheimer's disease and Parkinson's disease. In addition, the CHAT protein may also be a drug target for the treatment of these diseases.

Customer Reviews (3)

Write a review
Reviews
10/15/2022

    The resolution of CHAT in Western blot is high and can distinguish between similar protein molecular weights.

    02/14/2021

      This repeatability of CHAT is very good, which can greatly reduce the experimental error and allow me to evaluate the experimental results more accurately.

      04/25/2019

        Stability of CHAT is so good that a variety of experimental manipulations can be performed without concern for changes and effects on its properties.

        Ask a Question for All CHAT Products

        Required fields are marked with *

        My Review for All CHAT Products

        Required fields are marked with *

        0

        Inquiry Basket

        cartIcon
        logo

        FOLLOW US

        Terms and Conditions        Privacy Policy

        Copyright © 2024 Creative BioMart. All Rights Reserved.

        Contact Us

        • /

        Stay Updated on the Latest Bioscience Trends