Recombinant Human CHAT protein, His-tagged
Cat.No. : | CHAT-172H |
Product Overview : | Recombinant Human CHAT(Asp517~Pro732) fused with His tag at N-terminal was expressed in E. coli. |
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- Gene Information
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Description : | This gene encodes an enzyme which catalyzes the biosynthesis of the neurotransmitter acetylcholine. This gene product is a characteristic feature of cholinergic neurons, and changes in these neurons may explain some of the symptoms of Alzheimer's disease. Polymorphisms in this gene have been associated with Alzheimer's disease and mild cognitive impairment. Mutations in this gene are associated with congenital myasthenic syndrome associated with episodic apnea. Multiple transcript variants encoding different isoforms have been found for this gene, and some of these variants have been shown to encode more than one isoform. |
Source : | E. coli |
Species : | Human |
Tag : | His |
Form : | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300 |
Molecular Mass : | 28.0kDa |
Protein length : | Asp517~Pro732 |
Endotoxin : | <1.0EU per 1µg (determined by the LAL method) |
Purity : | > 95% |
Applications : | Positive Control; Immunogen; SDS-PAGE; WB. If bio-activity of the protein is needed, please check active protein |
Stability : | The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 centigrade for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition. |
Storage : | Avoid repeated freeze/thaw cycles. Store at 2-8 centigrade for one month. Aliquot and store at -80 centigrade for 12 months. |
Reconstitution : | Reconstitute in PBS or others. |
Gene Name : | CHAT choline O-acetyltransferase [ Homo sapiens ] |
Official Symbol : | CHAT |
Synonyms : | CHAT; choline O-acetyltransferase; choline acetyltransferase; choline acetylase; acetyl CoA:choline O-acetyltransferase; CMS1A; CMS1A2; CHOACTASE; |
Gene ID : | 1103 |
mRNA Refseq : | NM_001142929 |
Protein Refseq : | NP_001136401 |
MIM : | 118490 |
UniProt ID : | P28329 |
Chromosome Location : | 10q11.2 |
Pathway : | Acetylcholine Neurotransmitter Release Cycle, organism-specific biosystem; Acetylcholine Synthesis, organism-specific biosystem; Biogenic Amine Synthesis, organism-specific biosystem; Cholinergic synapse, organism-specific biosystem; Glycerophospholipid metabolism, organism-specific biosystem; Glycerophospholipid metabolism, conserved biosystem; Neuronal System, organism-specific biosystem; |
Function : | choline O-acetyltransferase activity; choline binding; transferase activity, transferring acyl groups; |
Products Types
◆ Recombinant Protein | ||
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CHAT-1021H | Recombinant Human CHAT Protein (Met1-Pro630), N-His tagged | +Inquiry |
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Related Gene
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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Q&As (6)
Ask a questionThe activity of the CHAT protein can be modulated by drugs or other small molecule compounds to treat neurological disorders associated with CHAT protein abnormalities. For example, some drugs can promote the expression and activity of the CHAT protein, thereby increasing acetylcholine synthesis.
Expression level of CHAT protein is regulated by a variety of factors, including gene expression, post-transcriptional modifications, nutritional status, etc. Some drugs or other small molecule compounds can also affect their expression levels by regulating gene expression or post-transcriptional modifications.
Some studies have shown that mutations or abnormal expression of the CHAT protein may be related to neurological diseases, psychiatric diseases, etc. However, further research is needed to confirm the association.
CHAT protein has some association with other neurotransmitters or signaling molecules. For example, it can interact with cholinergic neurons to co-regulate acetylcholine synthesis and release. In addition, it can interact with other neurotransmitters or signaling molecules such as dopamine, glutamate, etc.
The occurrence, progression and prognosis of neurological diseases can be assessed by detecting characteristics such as the expression level, activity and interaction of CHAT protein with other proteins. These features can also be used to develop new biomarkers and drug targets.
Some studies have shown that abnormal expression of CHAT protein may be related to the occurrence and progression of some neurological diseases, such as Alzheimer's disease and Parkinson's disease. In addition, the CHAT protein may also be a drug target for the treatment of these diseases.
Customer Reviews (3)
Write a reviewThe resolution of CHAT in Western blot is high and can distinguish between similar protein molecular weights.
This repeatability of CHAT is very good, which can greatly reduce the experimental error and allow me to evaluate the experimental results more accurately.
Stability of CHAT is so good that a variety of experimental manipulations can be performed without concern for changes and effects on its properties.
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