Active Recombinant Human CHEK1 Protein, GST-tagged

• Cat.No.: CHEK1-229H
• Product Overview: Recombinant human CHK1 (AAM78553.1) (Met1-Thr476) was fused with the GST tag at the N-terminus.

Specification

• Source: Baculovirus-Insect Cells
• Species: Human
• Tag: GST
• Predicted N Terminal: Met
• Form: Supplied as sterile 20mM Tris, 500mM NaCl, pH 7.4, 10% glycerol, 2mM GSH.
• Bio-activity: The specific activity was determined to be 32 nmol/min/mg using CHKtide peptide (KKKVSRSGLYRSPSMPENLNRPR) as substrate.
• Molecular Mass: The recombinant human CHK1/GST chimera consists of 700 amino acids and predicts a molecular mass of 80.7kDa. It migrates as an approxiamtely 80 KDa band as predicted in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
• Purity: >95 % as determined by SDS-PAGE.
• Stability: Samples are stable for up to twelve months from date of receipt at -70ºC.
• Storage: Store it under sterile conditions at -20ºC~-70ºC. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

Gene Information

• Gene Name: CHEK1 checkpoint kinase 1 [ Homo sapiens ]
• Official Symbol: CHEK1
• Synonyms: CHK1; CHK1 checkpoint homolog; Checkpoint, S. pombe, homolog of, 1; Chk1-S; cell cycle checkpoint kinase; checkpoint kinase-1
• Gene ID: 1111
• mRNA Refseq: NM_001114121
• Protein Refseq: NP_001107593
• MIM: 603078
• UniProt ID: O14757
• Chromosome Location: 11q24.2
• Pathway: Activation of ATR in response to replication stress, organism-specific biosystem; Cell cycle, conserved biosystem; DNA Double-Strand Break Repair, organism-specific biosystem