Recombinant Human CYP2C9/NADPH reductase protein

• Cat.No.: CYP2C9-35H
• Product Overview: This is a permeabilized and stabilized dried yeast powder preparation containing recombinant human CYP2C9 and recombinant human P450 NADPH oxidoreductase co-expressed in the same preparation.

Specification

• Source: Pichia pastoris
• Species: Human
• Form: Lyophilized powder
• Molecular Mass: 55 Kda
• Characteristic: - Co-expresses human CYP2C9 and human P450 oxidoreductase (hCPR) allowing easy reconstitution of the endogenous system- Useful for all the applications as a baculosome system but more cost-effective- Easy to handle dry powder- Very stable in dry form and active in multiple buffers and solvents during long incubations- Addition of NADP+ & Glucose-6-Phosphate are not essential (but will boost T3activity)- After the reaction, the protein can be pelleted at relatively low speeds- Clean HPLC profiles for easy metabolite ID and purification- Highly controlled production process for lot-to-lot reproducibility
• Notes: Centrifuge the vial prior to opening
• Storage: Stable for 1 year as supplied. Store dry powder at -20 centigrade. Reconstitute in 25 mM Potassium Phosphate buffer, pH 7.7. Not recommended to be stored as solution. If storing as solution, aliquot and store at -80 centigrade. Avoid repeated freeze/thaw cycles and use aliquots within one month (the human CYP2C9 will lose approximately 10% activity per week when stored at -80 centigrade). Thaw aliquots rapidly at 37 centigrade and place on ice until use (thawed aliquots should be used within 4 hours).
• Reconstitution: 25 mM Potassium Phosphate buffer, pH 7.7

Gene Information

• Gene Name: CYP2C9 cytochrome P450, family 2, subfamily C, polypeptide 9 [ Homo sapiens ]
• Official Symbol: CYP2C9
• Synonyms: CYP2C9; cytochrome P450, family 2, subfamily C, polypeptide 9; CYP2C10, cytochrome P450, subfamily IIC (mephenytoin 4 hydroxylase), polypeptide 9; cytochrome P450 2C9; P450IIC9; cytochrome P-450MP; cytochrome P450 PB-1; microsomal monooxygenase; xenobiotic monooxygenase; flavoprotein-linked monooxygenase; cytochrome P-450 S-mephenytoin 4-hydroxylase; CPC9; CYP2C; CYP2C10; CYPIIC9; MGC88320; MGC149605
• Gene ID: 1559
• mRNA Refseq: NM_000771
• Protein Refseq: NP_000762
• MIM: 601130
• UniProt ID: P11712
• Chromosome Location: 10q24.1
• Pathway: Arachidonate Epoxygenase / Epoxide Hydrolase, organism-specific biosystem; Arachidonic acid metabolism, organism-specific biosystem; Arachidonic acid metabolism, conserved biosystem; Biological oxidations, organism-specific biosystem; Cytochrome P450 - arranged by substrate type, organism-specific biosystem; Drug metabolism - cytochrome P450, organism-specific biosystem; Drug metabolism - cytochrome P450, conserved biosystem
• Function: (R)-limonene 6-monooxygenase activity; (S)-limonene 6-monooxygenase activity; (S)-limonene 7-monooxygenase activity; caffeine oxidase activity; drug binding; electron carrier activity; heme binding; metal ion binding; monooxygenase activity; monooxygenase activity; oxidoreductase activity; oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen; steroid hydroxylase activity