Active Recombinant Human DAPK1 Protein, His-tagged

• Cat.No.: DAPK1-2336H
• Product Overview: Human DAPK1 (NP_004929.1, 1 a.a. - 289 a.a.) partial recombinant protein with His tag expressed in Baculovirus infected Sf21 cells.

Specification

• Description: Death-associated protein kinase 1 is a positive mediator of gamma-interferon induced programmed cell death. DAPK1 encodes a structurally unique 160-kD calmodulin dependent serine-threonine kinase that carries 8 ankyrin repeats and 2 putative P-loop consensus sites. It is a tumor suppressor candidate. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2013]
• Source: Insect Cells
• Species: Human
• Tag: His
• Form: Liquid
• Bio-activity: The activity was measured by off-chip mobility shift assay. The enzyme was incubated with fluorescence-labeled substrate and Mg (or Mn)/ATP. The phosphorylated and unphosphorylated substrates were separated and detected by LabChip™3000.Substrate : DAPK1tide. ATP: 100 µM.
• Molecular Mass: 60 kDa
• Purity: 94 % by SDS-PAGE/CBB staining.
• Applications: Functional Study
  SDS-PAGE
• Storage: Store at -80 centigrade. Aliquot to avoid repeated freezing and thawing.
• Storage Buffer: In 50 mM Tris-HCl, 150 mM NaCl, pH 7.5 (0.1% CHAPS, 1 mM DTT, 10% glycerol)

Gene Information

• Gene Name: DAPK1 death-associated protein kinase 1 [ Homo sapiens ]
• Official Symbol: DAPK1
• Synonyms: DAPK1; death-associated protein kinase 1; DAPK; DAP kinase 1; DKFZp781I035
• Gene ID: 1612
• mRNA Refseq: NM_004938
• Protein Refseq: NP_004929
• MIM: 600831
• UniProt ID: P53355