Recombinant Human DEFB4A protein, His & GST-tagged

• Cat.No.: DEFB4A-632H
• Product Overview: Recombinant Human DEFB4A aa. (Gly24~Pro64) fused with N-terminal His & GST tag was produced in E. coli cells.

Specification

• Description: Defensins form a family of microbicidal and cytotoxic peptides made by neutrophils. Members of the defensin family are highly similar in protein sequence. This gene encodes defensin, beta 4, an antibiotic peptide which is locally regulated by inflammation.
• Source: E. coli
• Species: Human
• Tag: His & GST
• Form: Freeze-dried powder
• Molecular Mass: 35kDa as determined by SDS-PAGE reducing conditions
• Protein length: Gly24~Pro64
• Endotoxin: <1.0EU per 1µg (determined by the LAL method)
• Purity: >95%
• Characteristic: The isoelectric point is 8.
• Applications: SDS-PAGE; WB; ELISA; IP.
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
• Storage buffer: 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5%Trehalose and Proclin300.
• Reconstitution: Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Gene Information

• Gene Name: DEFB4A defensin beta 4A [ Homo sapiens (human) ]
• Official Symbol: DEFB4A
• Synonyms: DEFB4A; defensin beta 4A; BD-2; SAP1; DEFB2; DEFB4; HBD-2; DEFB-2; DEFB102; beta-defensin 4A; beta defensin-2; defensin, beta 2; defensin, beta 4; skin-antimicrobial peptide 1
• Gene ID: 1673
• mRNA Refseq: NM_004942.3
• Protein Refseq: NP_004933.1
• UniProt ID: O15263