Recombinant Human DPM1 Protein, GST-tagged

• Cat.No.: DPM1-2836H
• Product Overview: Human DPM1 full-length ORF ( AAH08466.1, 1 a.a. - 260 a.a.) recombinant protein with GST-tag at N-terminal.

Specification

• Description: Dolichol-phosphate mannose (Dol-P-Man) serves as a donor of mannosyl residues on the lumenal side of the endoplasmic reticulum (ER). Lack of Dol-P-Man results in defective surface expression of GPI-anchored proteins. Dol-P-Man is synthesized from GDP-mannose and dolichol-phosphate on the cytosolic side of the ER by the enzyme dolichyl-phosphate mannosyltransferase. Human DPM1 lacks a carboxy-terminal transmembrane domain and signal sequence and is regulated by DPM2. Mutations in this gene are associated with congenital disorder of glycosylation type Ie. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Nov 2015]
• Source: Wheat Germ
• Species: Human
• Tag: GST
• Molecular Mass: 56 kDa
• AA Sequence: MASLEVSRGPRRSRRELEVRSPRQN KYSVLLPTYNERENLPLIVWLLVKS FSESGINYEIIIIDDGSPDGTRDVA EQLEKIYGSDRILLRPREKKLGLGT AYIHGMKHATGNYIIIMDADLSHHP KFIPEFIRKQKEGNFDIVSGTRYKG NGGVYGWDLKRKIISRGANFLTQIL LRPGASDLTGSFRLYRKEVLEKLIE KCVSKGYVFQMEMIVRARQMNYTIG EVPISFVDRVYGESKLGGNEIVSFL KGLLTLFATT
• Applications: Enzyme-linked Immunoabsorbent Assay
  Western Blot (Recombinant protein)
  Antibody Production
  Protein Array
• Notes: Best use within three months from the date of receipt of this protein.
• Storage: Store at -80 centigrade. Aliquot to avoid repeated freezing and thawing.
• Storage Buffer: 50 mM Tris-HCI, 10 mM reduced Glutathione, pH=8.0 in the elution buffer.

Gene Information

• Gene Name: DPM1 dolichyl-phosphate mannosyltransferase polypeptide 1, catalytic subunit [ Homo sapiens ]
• Official Symbol: DPM1
• Synonyms: DPM1; dolichyl-phosphate mannosyltransferase polypeptide 1, catalytic subunit; dolichol-phosphate mannosyltransferase; CDGIE; MPDS; DPM synthase; MPD synthase; mannose-P-dolichol synthase; dolichol-phosphate mannose synthase; dolichol monophosphate mannose synthase; dolichyl-phosphate beta-D-mannosyltransferase
• Gene ID: 8813
• mRNA Refseq: NM_003859
• Protein Refseq: NP_003850
• MIM: 603503
• UniProt ID: O60762