Recombinant Human ELANE Protein, GST-tagged

• Cat.No.: ELANE-1197H
• Product Overview: Recombinant Human ELANE Protein (30-267aa) was expressed in E. coli with N-terminal GST-tag.

Specification

• Description: Elastases form a subfamily of serine proteases that hydrolyze many proteins in addition to elastin. Humans have six elastase genes which encode structurally similar proteins. The encoded preproprotein is proteolytically processed to generate the active protease. Following activation, this protease hydrolyzes proteins within specialized neutrophil lysosomes, called azurophil granules, as well as proteins of the extracellular matrix. The enzyme may play a role in degenerative and inflammatory diseases through proteolysis of collagen-IV and elastin. This protein also degrades the outer membrane protein A (OmpA) of E. coli as well as the virulence factors of such bacteria as Shigella, Salmonella and Yersinia. Mutations in this gene are associated with cyclic neutropenia and severe congenital neutropenia (SCN). This gene is present in a gene cluster on chromosome 19.
• Source: E. coli
• Species: Human
• Tag: GST
• Form: Tris-based buffer, 50% glycerol.
• Molecular Mass: 52.6 kDa
• AA Sequence: IVGGRRARPHAWPFMVSLQLRGGHF CGATLIAPNFVMSAAHCVANVNVRA VRVVLGAHNLSRREPTRQVF
  AVQRIFENGYDPVNLLNDIVILQLN GSATINANVQVAQLPAQGRRLGNGV QCLAMGWGLLGRNRGIASVL
  QELNVTVVTSLCRRSNVCTLVRGRQ AGVCFGDSGSPLVCNGLIHGIASFV RGGCASGLYPDAFAPVAQFV
  NWIDSIIQRSEDNPCPHPRDPDPAS RTH
• Purity: Greater than 90% as determined by SDS-PAGE.
• Storage: The shelf life of liquid form is 6 months at -20 centigrade/-80 centigrade. The shelf life of lyophilized form is 12 months at -20 centigrade/-80 centigrade.

Gene Information

• Gene Name: ELANE elastase, neutrophil expressed [ Homo sapiens ]
• Official Symbol: ELANE
• Synonyms: GE; NE; HLE; HNE; ELA2; SCN1; PMN-E
• Gene ID: 1991
• mRNA Refseq: NM_001972.2
• Protein Refseq: NP_001963.1
• MIM: 130130
• UniProt ID: P08246