Recombinant Human ENO1 Protein, GST-tagged

• Cat.No.: ENO1-3309H
• Product Overview: Human ENO1 full-length ORF ( AAH11130.1, 1 a.a. - 434 a.a.) recombinant protein with GST-tag at N-terminal.

Specification

• Description: This gene encodes alpha-enolase, one of three enolase isoenzymes found in mammals. Each isoenzyme is a homodimer composed of 2 alpha, 2 gamma, or 2 beta subunits, and functions as a glycolytic enzyme. Alpha-enolase in addition, functions as a structural lens protein (tau-crystallin) in the monomeric form. Alternative splicing of this gene results in a shorter isoform that has been shown to bind to the c-myc promoter and function as a tumor suppressor. Several pseudogenes have been identified, including one on the long arm of chromosome 1. Alpha-enolase has also been identified as an autoantigen in Hashimoto encephalopathy. [provided by RefSeq, Jan 2011]
• Source: Wheat Germ
• Species: Human
• Tag: GST
• Molecular Mass: 73.48 kDa
• AA Sequence: MSILKIHAREIFDSRGNPTVEVDLF TSKGLFRAAVPSGASTGIYEALELR DNDKTRYMGKGVSKAVEHINKTIAP ALVSKKLNVTEQEKIDKLMIEMDGT ENKSKFGANAILGVSLAVCKAGAVE KGVPLYRHIADLAGNSEVILPVPAF NVINGGSHAGNKLAMQEFMILPVGA ANFREAMRIGAEVYHNLKNVIKEKY GKDATNVGDEGGFAPNILENKEGLE LLKTAIGKAGYTDKVVIGMDVAASE FFRSGKYDLDFKSPDDPSRYISPDQ LADLYKSFIKDYPVVSIEDPFDQDD WGAWQKFTASAGIQVVGDDLTVTNP KRIAKAVNEKSCNCLLLKVNQIGSV TESLQACKLAQANGWGVMVSHRSGE TEDTFIADLVVGLCTGQIKTGAPCR SERLAKYNQLLRIEEGLGSKAKFAG RNFRNPLAK
• Applications: Enzyme-linked Immunoabsorbent Assay
  Western Blot (Recombinant protein)
  Antibody Production
  Protein Array
• Notes: Best use within three months from the date of receipt of this protein.
• Storage: Store at -80 centigrade. Aliquot to avoid repeated freezing and thawing.
• Storage Buffer: 50 mM Tris-HCI, 10 mM reduced Glutathione, pH=8.0 in the elution buffer.

Gene Information

• Gene Name: ENO1 enolase 1, (alpha) [ Homo sapiens ]
• Official Symbol: ENO1
• Synonyms: ENO1; enolase 1, (alpha); ENO1L1, MPB1; alpha-enolase; c-myc promoter-binding protein-1; MBP 1; PPH; alpha-enolase; enolase-alpha; tau-crystallin; non-neural enolase; alpha enolase like 1; phosphopyruvate hydratase; plasminogen-binding protein; MYC promoter-binding protein 1; 2-phospho-D-glycerate hydro-lyase; NNE; MPB1; ENO1L1
• Gene ID: 2023
• mRNA Refseq: NM_001201483
• Protein Refseq: NP_001188412
• MIM: 172430
• UniProt ID: P06733