"ERF1" Related Products

Recombinant Human ERF1 protein, GST-tagged

Cat.No.: ERF1-317H
Product Overview: Recombinant human ERF1 (2-end) protein was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.
Description: Eukaryotic peptide chain release factor subunit 1 (eRF1) directs the termination of nascent peptide synthesis (translation) in response to the termination codons UAA, UAG and UGA. Component of the transient SURF complex which recruits UPF1 to stalled ribosomes in the context of nonsense-mediated decay (NMD) of mRNAs containing premature stop codons.
Source: Insect cell
Species: Human
Tag : GST
Form: 50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol
Molecular Mass: 74 kDa
Purity: The purity of ERF1 was determined to be >95% by densitometry.
Applications: Enzyme Assay; WB
Stability: 1 year at -70 centigrade
Storage: Store product at -70 centigrade. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Concentration: 0.1 μg/μL
Gene Name: eukaryotic translation termination factor 1 [ Homo sapiens (human) ]
Official Symbol: ETF1
Synonyms: ERF; RF1; ERF1; TB3-1; D5S1995; SUP45L1
Gene ID: 2107
mRNA Refseq: NM_004730
Protein Refseq: NP_004721
MIM: 600282
UniProt ID: P62495

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Optional requirements on this protein    +Expand
C-fusion    N-fusion   Non-tagged
His    GST   Fc   Others
<1.0 eu/μg    <0.1 eu/μg   <0.01 eu/μg   Not required
Monomer Isolation    Dimer Isolation    Not required
>80% by SDS-PAGE    >90% by SDS-PAGE   >95% by SDS-PAGE   Others

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