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Recombinant Human FAS protein, His-tagged

Cat.No. : FAS-614H
Product Overview : Recombinant Human FAS (P25445) aa. (Gln26~Asn173) fused with N-terminal His tag was produced in E. coli cells.
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  • Gene Information
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Description : The protein encoded by this gene is a member of the TNF-receptor superfamily. This receptor contains a death domain. It has been shown to play a central role in the physiological regulation of programmed cell death, and has been implicated in the pathogenesis of various malignancies and diseases of the immune system. The interaction of this receptor with its ligand allows the formation of a death-inducing signaling complex that includes Fas-associated death domain protein (FADD), caspase 8, and caspase 10. The autoproteolytic processing of the caspases in the complex triggers a downstream caspase cascade, and leads to apoptosis. This receptor has been also shown to activate NF-kappaB, MAPK3/ERK1, and MAPK8/JNK, and is found to be involved in transducing the proliferating signals in normal diploid fibroblast and T cells. Several alternatively spliced transcript variants have been described, some of which are candidates for nonsense-mediated mRNA decay (NMD). The isoforms lacking the transmembrane domain may negatively regulate the apoptosis mediated by the full length isoform.
Source : E. coli
Species : Human
Tag : His
Form : Freeze-dried powder
Molecular Mass : Predicted Molecular Mass: 18.2kDa
Protein length : Gln26~Asn173 (Accession # P25445)
Endotoxin : <1.0EU per 1µg (determined by the LAL method)
Purity : > 95%
Characteristic : The isoelectric point is 6.7.
Applications : SDS-PAGE; WB; ELISA; IP.
Stability : The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Storage : Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
Storage buffer : PBS, pH7.4, containing 5% sucrose, 0.01% sarcosyl.
Reconstitution : Reconstitute in sterile PBS, pH7.2-pH7.4
Gene Name : FAS Fas cell surface death receptor [ Homo sapiens (human) ]
Official Symbol : FAS
Synonyms : FAS; Fas cell surface death receptor; APT1; CD95; FAS1; APO-1; FASTM; ALPS1A; TNFRSF6; tumor necrosis factor receptor superfamily member 6; APO-1 cell surface antigen; CD95 antigen; FASLG receptor; Fas (TNF receptor superfamily, member 6); Fas AMA; TNF receptor superfamily member 6; apoptosis antigen 1; apoptosis signaling receptor FAS; apoptosis-mediating surface antigen FAS; mutant tumor necrosis receptor superfamily member 6; tumor necrosis factor receptor superfamily, member 6
Gene ID : 355
mRNA Refseq : NM_000043.5
Protein Refseq : NP_000034.1
UniProt ID : P25445

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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Q&As (6)

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Does FAS protein play a role in cell signaling? 10/30/2022

FAS protein plays an important role in cell signaling, it can activate downstream signaling pathways, such as death signaling pathway and survival signaling pathway, by binding to its ligand and transmitting signals.

Why apply knowledge of FAS proteins to clinical practice? 06/27/2022

It can provide a reference for clinical practice and guide the diagnosis, treatment and prevention of diseases by detecting the expression level of FAS protein and understanding its mechanism of action.

How can diseases associated with FAS protein be prevented and treated? 05/13/2022

FAS protein-related diseases can be prevented and treated by regulating the expression level of FAS protein and inhibiting its signaling pathway.

How can gene knockout or knockdown techniques be used to study the function of FAS proteins? 03/31/2021

Gene knockout or knockdown techniques can be used to study the function and mechanism of action of FAS proteins. By knocking out or knocking down the FAS gene, changes in processes such as apoptosis and immune response can be observed to further understand its function and mechanism of action.

What is the role of FAS protein in apoptosis? 09/29/2020

FAS protein can bind to its ligand, activate the death signaling pathway, and induce apoptosis.

How can FAS protein be used to predict disease prognosis? 06/19/2020

The expression level and activity of FAS protein can be measured to predict the prognosis and development trend of certain diseases.

Customer Reviews (3)

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Reviews
09/07/2022

    When experimenting with FAS, the results are very reproducible.

    07/29/2022

      The stability of FAS products allows them to cope with different experimental conditions and modes of operation.

      09/20/2021

        FAS has high specific activity and can exert strong biological effect at low concentration.

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