Active Recombinant Human FGFR3 protein, Fc-tagged

• Cat.No.: FGFR3-751H
• Product Overview: Recombinant Human FGFR3 protein(Glu 23 - Gly 375), fused with Fc tag, was expressed in HEK293.

Specification

• Source: HEK293
• Species: Human
• Tag: Fc
• Form: Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, 25 mM Arginine, 150 mM NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization.
• Bio-activity: Immobilized Human FGF basic, premium grade at 5 μg/mL (100 μL/well) can bind Human FGF R3 (IIIc), Fc Tag with a linear range of 1-39 ng/mL.Immobilized Biotinylated Human FGF basic, Avitag,His Tag at 1 μg/mL (100 μL/well) on streptavidin precoated (0.5 μg/well) plate can bind Human FGF R3 (IIIc), Fc Tag with a linear range of 2-39 ng/mL.
• Molecular Mass: This protein carries a human IgG1 Fc tag at the C-terminus.The protein has a calculated MW of 64.6 kDa. The protein migrates as 80-90 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Protein Length: Glu23-Gly375
• Endotoxin: Less than 1.0 EU per μg by the LAL method.
• Purity: >95% as determined by SDS-PAGE.>90% as determined by SEC-MALS.
• Storage: For long term, the product should be stored at lyophilized state at -20°C or lower.Please protect from light and avoid repeated freeze-thaw cycles.This product is stable after at: -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: FGFR3 fibroblast growth factor receptor 3 [ Homo sapiens ]
• Official Symbol: FGFR3
• Synonyms: FGFR3; fibroblast growth factor receptor 3; ACH, achondroplasia, thanatophoric dwarfism; CD333; CEK2; JTK4; FGFR-3; tyrosine kinase JTK4; hydroxyaryl-protein kinase; ACH; HSFGFR3EX
• Gene ID: 2261
• mRNA Refseq: NM_000142
• Protein Refseq: NP_000133
• MIM: 134934
• UniProt ID: P22607