Recombinant Human GAK protein, GST-tagged

• Cat.No.: GAK-15H
• Product Overview: Recombinant Human GAK(1-396 aa) fused with GST tag at N-terminal was expressed in Insect cells.

Specification

• Description: Cyclin-G-associated kinase (GAK) is a serine/threonine protein kinase and was originally identified as a protein that is associated with cyclin G. However, neither CDK5 nor GAK kinase activity is activated by cyclin G nor does GAK affect CDK5 kinase activity. Seems to act as an auxilin homolog that is involved in the uncoating of clathrin-coated vesicles by Hsc70 in non-neuronal cells. Expression oscillates slightly during the cell cycle, peaking at G1.
• Source: Insect cells
• Species: Human
• Tag: GST
• Form: 50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol.
• Molecular Mass: ~68 kDa
• Purity: >95%
• Applications: Kinase Assay
• Stability: 1 year at -80 centigrade from the date of shipment
• Storage: Store at -70 centigrade. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
• Concentration: 0.1μg/μl

Gene Information

• Gene Name: GAK cyclin G associated kinase [ Homo sapiens ]
• Official Symbol: GAK
• Synonyms: GAK; cyclin G associated kinase; cyclin-G-associated kinase; auxilin 2; DNAJC26; auxilin-2; DNAJ26; FLJ16629; FLJ40395; MGC99654
• Gene ID: 2580
• mRNA Refseq: NM_005255
• Protein Refseq: NP_005246
• MIM: 602052
• UniProt ID: O14976
• Chromosome Location: 4p16
• Pathway: Clathrin derived vesicle budding, organism-specific biosystem; Golgi Associated Vesicle Biogenesis, organism-specific biosystem; Membrane Trafficking, organism-specific biosystem; trans-Golgi Network Vesicle Budding, organism-specific biosystem
• Function: ATP binding; cyclin binding; heat shock protein binding; nucleotide binding; protein kinase activity; protein serine/threonine kinase activity; transferase activity, transferring phosphorus-containing groups