| Description : |
Interleukin 16 (IL16) is a pleiotrophic cytokine derived from CD8+ T-cells that stimulates chemotaxis in CD4+ lymphocytes, monocytes, and eosinophils. It primes CD4+ T-cells for IL2 and IL15 responsiveness, induces T-lymphocyte Interleukin 2 (IL2) receptor expression, and inhibits HIV replication. The signalling process of IL16 is mediated by CD4, and undergoes proteolysis to yield two functional proteins. IL16 isoform 1 has the possible function of acting as a scaffolding protein to anchor ion channels in the membrane, while isoform 3 is involved in cell cycle progression in T-cells and transcriptional regulation of SKP2. IL16 mRNA expression levels have been found in various tissues such as spleen, bone marrow, thymus, and lymph nodes. |
| Source : |
E. coli |
| Species : |
Human |
| Form : |
Lyophilized from a 0.2 μm filtered solution in PBS. |
| Bio-activity : |
The ED50 is 7.5-37.5 ng/mL as determined by its ability to chemoattract human CD4+ T lymphocytes (at a concentration range of 1.0-100.0 ng/mL). |
| Molecular Mass : |
13.5 kDa |
| Endotoxin : |
<1.0 EU/μg of recombinant protein as determined by the LAL method. |
| Purity : |
>95% |
| Applications : |
Cell Assay |
| Storage : |
The lyophilized protein is stable for at least one year from date of receipt at -70 centigrade. Upon reconstitution, this cytokine can be stored in working aliquots at 2 centigrade - 8 centigrade for one month, or at -20 centigrade for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles. |
| Reconstitution : |
Reconstitute in sterile PBS at 5 μg/Ml |
