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Active Recombinant Human IL19 protein, His-tagged

Cat.No. : IL19-482H
Product Overview : Recombinant Human IL19 protein(Met1-Ala177)(AAF06663.1), fused with His tag, was expressed in HEK293.
  • Specification
  • Gene Information
  • Related Products
Source : HEK293
Species : Human
Tag : His
Form : Lyophilized from sterile PBS, pH 7.4.Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
Bio-activity : Using the Octet RED System, the affinity constant (Kd) of IL-19 Protein, Human, Recombinant (His Tag) bound Anti-IL-19 Antibody was 0.1 nM.
Molecular Mass : The recombinant human IL19 consists 164 amino acids and predicts a molecular mass of 19.2 kDa.
Protein Length : Met1-Ala177
Endotoxin : < 1.0 EU per μg protein as determined by the LAL method.
Purity : > 95 % as determined by SDS-PAGE.
Storage : Samples are stable for up to twelve months from date of receipt at -20°C to -80°C
Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution : It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.
Gene Name : IL19 interleukin 19 [ Homo sapiens ]
Official Symbol : IL19
Synonyms : IL19; interleukin 19; interleukin-19; IL 10C; IL 19; MDA1; melanoma differentiation associated protein like protein; NG.1; ZMDA1; melanoma differentiation associated protein-like protein; melanoma differentiation-associated protein-like protein; IL-10C;
Gene ID : 29949
mRNA Refseq : NM_013371
Protein Refseq : NP_037503
MIM : 605687
UniProt ID : Q9UHD0

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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Q&As (10)

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How are advanced imaging methods, like confocal microscopy and flow cytometry, applied to visualize IL19-mediated cellular responses and receptor internalization? 11/26/2022

Confocal microscopy and flow cytometry visualize IL19-induced cellular responses and track receptor internalization upon ligand binding.

What methods are employed to explore IL19's effects on immune cell polarization, cytokine secretion, and its potential involvement in chronic inflammatory conditions? 06/28/2022

Functional assays using immune cell cultures and cytokine profiling assays dissect IL19's impact on immune cell polarization and cytokine secretion.

What experimental strategies are utilized to investigate the binding affinities and stoichiometry of IL19 interactions with its cognate receptor IL20RA and potential co-receptor IL20RB? 03/30/2021

Biophysical techniques like surface plasmon resonance and analytical ultracentrifugation quantify IL19's binding to IL20RA and IL20RB and reveal receptor complex composition.

What techniques, such as surface plasmon resonance and isothermal titration calorimetry, are used to quantify IL19's binding kinetics to its receptor complexes? 08/14/2020

Biophysical techniques such as surface plasmon resonance and isothermal titration calorimetry quantify IL19's binding kinetics to its receptor complexes.

Could you elaborate on the use of CRISPR/Cas9-based knockout models and RNA interference to dissect IL19's role in specific cell types and tissues? 01/14/2020

CRISPR/Cas9 knockout models and siRNA-mediated knockdown in specific cell types unravel IL19's cell-specific roles.

What experimental methodologies, including neutralizing antibodies and receptor blockade, are employed to assess IL19's potential as a therapeutic target in inflammatory and autoimmune diseases? 12/18/2018

Neutralizing antibodies, receptor blockade experiments, and in vivo disease models evaluate IL19's suitability as a therapeutic target in inflammatory and autoimmune diseases.

How do researchers employ recombinant protein production techniques to generate bioactive IL19 for in vitro and in vivo functional studies? 11/08/2018

Recombinant protein expression in bacterial or mammalian systems followed by purification allows generation of functional IL19 for various experimental assays.

How are reporter gene assays, chromatin immunoprecipitation, and DNA-protein interaction techniques applied to uncover the transcriptional regulation of IL19 and its downstream targets? 11/03/2018

Reporter gene assays, ChIP, and DNA-protein interaction studies probe IL19's transcriptional regulation and interactions with specific DNA sequences.

How do researchers utilize animal models and patient samples to study IL19's contribution to skin disorders, such as psoriasis, and its therapeutic implications? 04/19/2017

Animal models and patient samples, combined with histopathology and molecular assays, shed light on IL19's involvement in skin disorders and its therapeutic potential.

Can you explain the experimental approaches to elucidate IL19's role in wound healing, tissue repair, and its impact on fibroblast and keratinocyte function? 02/18/2017

Animal wound healing models, in vitro cell migration assays, and co-culture experiments investigate IL19's role in tissue repair and its effects on fibroblast and keratinocyte function.

Customer Reviews (3)

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Reviews
02/22/2021

    High reproducibility.

    07/11/2020

      Suitable for Western blot.

      04/01/2019

        Effective in cell differentiation.

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