Recombinant Human KEAP1 protein, His-tagged

• Cat.No.: KEAP1-829H
• Product Overview: Recombinant Human KEAP1(a.a: 321-609) fused with His tag at N-terminal was expressed in E. coli.

Specification

• Description: This gene encodes a protein containing KELCH-1 like domains, as well as a BTB/POZ domain. Kelch-like ECH-associated protein 1 interacts with NF-E2-related factor 2 in a redox-sensitive manner and the dissociation of the proteins in the cytoplasm is followed by transportation of NF-E2-related factor 2 to the nucleus. This interaction results in the expression of the catalytic subunit of gamma-glutamylcysteine synthetase. Two alternatively spliced transcript variants encoding the same isoform have been found for this gene.
• Source: E. coli
• Species: Human
• Tag: His
• Form: 40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 200 mM imidazole, 3 mM DTT, 20% glycerol
• Molecular Mass: 32.6 kDa
• Purity: >90%
• Applications: Useful for studying protein binding and screening small molecules.
• Storage: > 6 months at –80 centigrade.
• Concentration: 3.6 mg/ml
• Warning: Avoid freeze/thaw cycles. Storing diluted protein is not recommended, if necessary, include carrier protein (BSA 0.1 – 0.5%).

Gene Information

• Gene Name: KEAP1 kelch-like ECH-associated protein 1 [ Homo sapiens ]
• Official Symbol: KEAP1
• Synonyms: KEAP1; kelch-like ECH-associated protein 1; INrf2; KIAA0132; KLHL19; MGC1114; MGC4407; MGC9454; MGC10630; MGC20887; kelch-like protein 19; cytosolic inhibitor of Nrf2
• Gene ID: 9817
• mRNA Refseq: NM_203500
• Protein Refseq: NP_987096
• MIM: 606016
• UniProt ID: Q14145
• Chromosome Location: 19p13.2
• Pathway: Adaptive Immune System, organism-specific biosystem; Antigen processing: Ubiquitination and Proteasome degradation, organism-specific biosystem; Class I MHC mediated antigen processing & presentation, organism-specific biosystem; Immune System, organism-specific biosystem; Keap1-Nrf2 Pathway, organism-specific biosystem
• Function: protein binding