Active Recombinant Human KITLG Protein, His-tagged, Biotinylated

• Cat.No.: KITLG-522H
• Product Overview: Biotinylated Recombinant Human KITLG Protein (Glu 26 - Ala 190) (AAH69797), fused with an Avi tag at the C-terminus, followed by a polyhistidine tag, was expressed in HEK293.

Specification

• Source: HEK293
• Species: Human
• Tag: His
• Predicted N Terminal: Glu 26
• Form: Lyophilized from sterile PBS, pH7.4, 10% trehalose.
• Bio-activity: Measured by its binding ability in a functional ELISA. Immobilized Human CD117, Fc Tag at 2μg/mL (100 μL/well) can bind Biotinylated Human SCF, His Tag with a linear range of 0.1-3.1 ng/mL.
• Molecular Mass: Recombinant Human KITLG Protein has a calculated MW of 21.7 kDa. The protein migrates as 26-38 kDa on a SDS-PAGE gel under reducing (R) condition due to different glycosylation.
• Protein length: Glu 26 - Ala 190
• Endotoxin: Less than 1.0 EU per μg by the LAL method.
• Purity: >95% as determined by SDS-PAGE.
• Stability: Samples are stable for up to twelve months from date of receipt at -70ºC.
• Storage: Store it under sterile conditions at -20ºC~-70ºC. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4℃ before opening to recover the entire contents.

Gene Information

• Gene Name: KITLG KIT ligand [ Homo sapiens ]
• Official Symbol: KITLG
• Synonyms: KITLG; KIT ligand; MGF; kit ligand; familial progressive hyperpigmentation 2; FPH2; Kitl; KL 1; mast cell growth factor; SCF; SF; steel factor; stem cell factor; c-Kit ligand; KL-1; SHEP7; kit-ligand; DKFZp686F2250
• Gene ID: 4254
• mRNA Refseq: NM_000899
• Protein Refseq: NP_000890
• MIM: 184745
• UniProt ID: P21583
• Chromosome Location: 12q22
• Pathway: C-MYB transcription factor network, organism-specific biosystem; Cytokine-cytokine receptor interaction, organism-specific biosystem; Cytokine-cytokine receptor interaction, conserved biosystem; Hematopoietic cell lineage, organism-specific biosystem; Hematopoietic cell lineage, conserved biosystem; Kit Receptor Signaling Pathway, organism-specific biosystem; Melanogenesis, organism-specific biosystem
• Function: cytokine activity; growth factor activity; protein binding; stem cell factor receptor binding