Recombinant Human LILRB3 protein, Fc & Avi-tagged, Biotinylated

• Cat.No.: LILRB3-0623H
• Product Overview: Biotinylated Human LILRB3, Fc Tag is expressed from human 293 cells (HEK293). It contains AA Gly 24 - Glu 443 (Accession # AAI12199). Biotinylation of this product is performed using Avitag technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin. The biotin to protein ratio is 0.5-1 as determined by the HABA assay.

Specification

• Description: Leukocyte immunoglobulin-like receptor subfamily B member 3 is also known as LILRB3,ILT-5 or CD85a. LILRB3 plays an role as receptor for class I MHC antigens, which activated upon coligation of LILRB3 and immune receptors, such as FCGR2B and the B-cell receptor. LILRB3 and LILRA6 represent a pair of inhibitory/activating receptors with identical extracellular domains and unknown ligands. LILRB3 can mediate inhibitory signaling via immunoreceptor tyrosine-based inhibition motifs in its cytoplasmic tail whereas LILRA6 can signal through association with an activating adaptor molecule, FcRγ.
• Source: HEK293
• Species: Human
• Tag: Fc & Avi
• Predicted N Terminal: Gly 24
• Form: Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, pH7.5, 10% trehalose.
• Molecular Mass: The protein has a calculated MW of 74.5 kDa. As a result of glycosylation, the protein migrates as 80-105 kDa under reducing (R) condition, and 150 kDa under non-reducing (NR) condition (SDS-PAGE).
• Protein length: Gly 24 - Glu 443
• Endotoxin: Less than 1.0 EU per μg by the LAL method.
• Purity: >95% as determined by SDS-PAGE.
• Storage: For long term storage, the product should be stored at lyophilized state at -20°C or lower. Please avoid repeated freeze-thaw cycles. No activity loss is observed after storage at: 4-8°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4℃ before opening to recover the entire contents.
• Shipping: Powder,RT

Gene Information

• Gene Name: LILRB3 leukocyte immunoglobulin like receptor B3 [ Homo sapiens (human) ]
• Official Symbol: LILRB3
• Synonyms: HL9; ILT5; LIR3; PIRB; CD85A; ILT-5; LIR-3; PIR-B; LILRA6; CD85 antigen-like family member A; immunoglobulin-like transcript 5; leucocyte Ig-like receptor B3; leukocyte immunoglobulin-like receptor, subfamily A (with TM domain), member 6; leukocyte; immunoglobulin-like receptor, subfamily B (with TM and ITIM domains), member 3; monocyte inhibitory receptor HL9; leukocyte immunoglobulin-like receptor subfamily B member 3
• Gene ID: 11025
• mRNA Refseq: NM_001081450.2
• Protein Refseq: NP_001074919.2