Recombinant Human NCOA1 protein, Biotinylated
Cat.No. : | NCOA1-373H |
Product Overview : | The His-tag biotinylated recombinant protein is purified by affinity chromatography in combination with FPLC columns. Then His-tag was removed by protease. |
- Specification
- Gene Information
- Related Products
Description : | Steroid receptor coactivator 1 (SRC1) is a transcriptional coactivoator that mediates the activating functions of many of the nuclear hormone receptors. It is also known as NCoA1 and is a member of the SRC/p160 coactivator family. SRC1 has been shown to be over expressed in some cancers. SRC1 is a 160 kDa protein that contains several LXXLL motifs, which are involved in nuclear receptor interaction. The region 627-786 contains 3 LXXLL motifs that are involved in interaction with nuclear hormone receptors and has been previously used in assays detecting ligand-dependent receptor-cofactor interactions. This protein does not contain His tag and will be useful for assays where a native untagged protein is desired. Lane 1 corresponds to 6His-tagged and biotinylated SRC-RID; Lane 2 corresponds to biotinylated SRC-RID without 6His tag. |
Source : | E. coli |
Species : | Human |
Form : | The protein is in 20mM Tris-HCl pH7.9,100mM NaCl, 0.2mM EDTA, 1mM DTT and 20% glycerol. |
Molecular Mass : | 19.4 kDa |
Protein length : | 627-786 aa |
AA Sequence : | SQTSHKLVQL LTTTAEQQLR HADIDTSCKD VLSCTGTSNS ASANSSGGSC PSSHSSLTERHKILHRLLQE GSPSDITTLS VEPDKKDSAS TSVSVTGQVQ GNSSIKLELD ASKKKESKDHQLLRYLLDKD EKDLRSTPNL SLDDVKVKVE KKEQMDPCNT |
Purity : | Purified biotinylated SRC1-RID is greater than 90% homogeneous based on SDS-PAGE analysis. |
Unit Definition : | 1 unit equals 1 nanogram of purified protein. 100 units are sufficient for a protein-protein interaction assay. |
Applications : | The biotinylated SCR1-RID (627-786) can be used for protein-protein interaction assays, particularly suitable for high throughput screening (HTS) assays. |
Storage : | Stored at -70 centigrade before use. Avoid repeated freeze thaw cycles. |
Gene Name : | NCOA1 nuclear receptor coactivator 1 [ Homo sapiens ] |
Official Symbol : | NCOA1 |
Synonyms : | NCOA1; nuclear receptor coactivator 1; bHLHe74; F SRC 1; KAT13A; NCoA 1; RIP160; SRC1; Hin-2 protein; PAX3/NCOA1 fusion protein; steroid receptor coactivator-1; renal carcinoma antigen NY-REN-52; class E basic helix-loop-helix protein 74; F-SRC-1; bHLHe42; MGC129719; MGC129720; |
Gene ID : | 8648 |
mRNA Refseq : | NM_003743 |
Protein Refseq : | NP_003734 |
MIM : | 602691 |
UniProt ID : | Q15788 |
Chromosome Location : | 2p23 |
Pathway : | Adipogenesis, organism-specific biosystem; Androgen Receptor Signaling Pathway, organism-specific biosystem; BMAL1:CLOCK/NPAS2 Activates Circadian ex |
Function : | DNA binding; androgen receptor binding; chromatin binding; enzyme binding; histone acetyltransferase activity; ligand-dependent nuclear receptor binding; ligand-dependent nuclear receptor transcription coactivator activity; nuclear hormone receptor binding; protein N-terminus binding; protein binding; signal transducer activity |
Products Types
◆ Recombinant Protein | ||
NCOA1-2481H | Recombinant Human NCOA1 Protein, His-tagged | +Inquiry |
NCOA1-5946M | Recombinant Mouse NCOA1 Protein, His (Fc)-Avi-tagged | +Inquiry |
NCOA1-29880TH | Recombinant Human NCOA1, His-tagged | +Inquiry |
NCOA1-29883TH | Recombinant Human NCOA1, FLAG-tagged | +Inquiry |
NCOA1-372H | Recombinant Human NCOA1 protein, His-tagged and Biotinylated | +Inquiry |
◆ Lysates | ||
NCOA1-3943HCL | Recombinant Human NCOA1 293 Cell Lysate | +Inquiry |
Related Gene
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
Inquiry
0
Inquiry Basket