Active Recombinant Human PCSK9 protein, Avi-His-tagged, Biotinylated

• Cat.No.: PCSK9-187H
• Product Overview: Biotinylated Recombinant Human PCSK9 protein(Gln 31 - Gln 692(D374Y)), fused with Avi and His tag, was expressed in HEK293.

Specification

• Source: HEK293
• Species: Human
• Tag: Avi-His
• Form: Lyophilized from 0.22 μm filtered solution in PBS, pH7.4, 10% trehalose.
• Bio-activity: Immobilized Human LDL R, His Tag at 2 μg/mL (100 μL/well) can bind Biotinylated Human PCSK9 (D374Y), Avitag,His Tag with a linear range of 0.2-8 ng/mL.
• Molecular Mass: This protein carries an Avi tag (Avitag) at the C-terminus, followed by a polyhistidine tag. This protein undergoes autocatalytic cleavage to release the pro-peptide and mature chain. The pro-peptide and mature chain are associated through non‑covalent interactions and with a calculated MW of 13.8 kDa and 59.9 kDa respectively. The protein migrates as 17 kDa and 60-65 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation. The D374Y mutation results in higher affinity of PCSK9 for LDLR.
• Protein Length: Gln 31 - Gln 692(D374Y)
• Endotoxin: Less than 1.0 EU per μg by the LAL method.
• Purity: >95% as determined by SDS-PAGE.
• Storage: For long term, the product should be stored at lyophilized state at -20°C or lower.Please avoid repeated freeze-thaw cycles. This product is stable after at:
  -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: PCSK9 proprotein convertase subtilisin/kexin type 9 [ Homo sapiens ]
• Official Symbol: PCSK9
• Synonyms: PCSK9; proprotein convertase subtilisin/kexin type 9; HCHOLA3, hypercholesterolemia, autosomal dominant 3; FH3; NARC 1; NARC1; NARC-1; HCHOLA3
• Gene ID: 353175
• MIM: 607786
• UniProt ID: Q8NBP7