Active Recombinant Human PFKFB3 protein, His-tagged

• Cat.No.: PFKFB3-24H
• Product Overview: Recombinant Human PFKFB3(Pro2-His520) fused with N-terminal Met and 6-His tag was expressed in E. coli.

Specification

• Description: 6-Phosphofructo-2-kinase/fructose-2,6-bisphosphatase 3 (PFKFB3) is involved in both the synthesis and degradation of fructose-2,6-bisphosphate, a regulatory molecule that controls the activity of the enzymes phosphofructokinase 1 (PFK-1) and fructose 1,6-bisphosphatase (FBPase-1) to regulate glycolysis and gluconeogenesis. PFKFB3 has a 6-phosphofructo-2-kinase activity that catalyzes the synthesis of fructose-2,6-bisphosphate, and a fructose-2,6-biphosphatase activity that catalyzes the degradation of fructose-2,6-bisphosphate. Recently, it is reported to be involved in vessel sprouting of endothelial cells (ECs). ECs rely on glycolysis rather than on oxidative phosphorylation for ATP production and that loss of the glycolytic activator PFKFB3 in ECs impairs vessel formation. PFKFB3 also plays a crucial role in the progression of cancerous cells by enabling their glycolytic pathways even under severe hypoxic conditions, which makes it a potential target for cancer therapy. The kinase activity of recombinant human PFKFB3 was assayed using a phosphatase-coupled method.
• Source: E. coli
• Species: Human
• Tag: His
• Predicted N Terminal: Met
• Form: Supplied as a 0.2 μm filtered solution in Tris, NaCl and DTT.
• Bio-activity: Measured by its ability to phosphorylate fructose-6-phosphate. The specific activity is >70 pmol/min/μg.
• Molecular Mass: Predicted Molecular Mass: 60 kDa;
  SDS-PAGE: 57 kDa, reducing conditions.
• Endotoxin: <1.0 EU per 1 μg of the protein by the LAL method.
• Purity: >85%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain at 5 μg per lane
• Storage: Avoid repeated freeze-thaw cycles.
  6 months from date of receipt, -70 centigrade as supplied.
  3 months, -70 centigrade under sterile conditions after opening.

Gene Information

• Gene Name: PFKFB3 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 3 [ Homo sapiens ]
• Official Symbol: PFKFB3
• Synonyms: PFKFB3; 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 3; 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 3; iPFK-2; PFK/FBPase 3; 6PF-2-K/Fru-2,6-P2ase 3; renal carcinoma antigen NY-REN-56; 6PF-2-K/Fru-2,6-P2ase brain/placenta-type isozyme; 6-phosphofructo-2-kinase/ fructose-2,6-bisphosphatase; 6-phosphofructo-2-kinase/fructose-2, 6-bisphosphatase; fructose-6-phosphate,2-kinase/fructose-2, 6-bisphosphatase; inducible 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase; PFK2; IPFK2; FLJ37326
• Gene ID: 5209
• mRNA Refseq: NM_001145443
• Protein Refseq: NP_001138915
• MIM: 605319
• UniProt ID: Q16875