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Home > Products > Recombinant Proteins > Recombinant Human RHOT1 protein, His-tagged

Recombinant Human RHOT1 protein, His-tagged

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Chicken(1)
Human(13)
Mouse(10)
Rat(3)
By Source
By Tag
His(3)
Cat.No.:
RHOT1-261H
Product Overview:
Recombinant Human RHOT1(Ile186 - Thr591) fused with His tag at N-terminal was expressed in E. coli.
Description:
Mitochondrial Rho GTPase 1 (also known as Miro1 or Rhot1) is a membrane associated GTPase with a predicted molecular weight of 71 kDa. Miro1 has been reported to directly associate with the KIF5 motor protein, allowing mitochondria to move along microtubules. A number of publications have identifed Miro1 as an in vivo substrate for the E3 Ubiquitin ligase Parkin (encoded by the PARK2 gene), an essential component of the cellular machinery that participates in the removal of damaged mitochondria. This protein is useful as an in vitro substrate for activated recombinant Parkin enzyme.
Source:
E. coli
Species:
Human
Tag :
His
Form:
X mg/ml (X μM) in 50 mM HEPES, 200 mM NaCl, 2 mM TCEP
Bio-activity:
Reaction Conditions will be optimized for each specific application. In a 50 μl reaction containing 1 μM activated Parkin E3 ligase, 1 μM His6-Miro1 is fully converted to Ubiquitinated forms in 60 minutes.
Molecular Mass:
Predicted Molecular Mass: 50 kDa
Purity:
>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Storage:
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -70 centigrade as supplied.
3 months, -70 centigrade under sterile conditions after opening.


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Optional requirements on this protein +Expand
C-fusion   N-fusion  Non-tagged
His   GST  Fc  Others
<1.0 eu/μg   <0.1 eu/μg  <0.01 eu/μg  Not required
Monomer Isolation   Dimer Isolation   Not required
>80% by SDS-PAGE   >90% by SDS-PAGE  >95% by SDS-PAGE  Others
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45-1 Ramsey Road, Shirley, NY 11967, USA
USA: 1-631-559-9269  1-631-448-7888
Europe: 44-207-048-3343
FAX: 1-631-938-8127
EMAIL: info@creative-biomart.com

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