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Recombinant Human ROR1 protein, His-tagged

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Cat.No.:
ROR1-1812H
Product Overview:
Human ROR1 (39-151, Ig-like domain) Protein (Human ROR1 (39-151, Ig-like domain), His Tag) Glu 39 - Gly 151 (Accession # Q01973) was produced in human 293 cells (HEK293).
Description:
Tyrosine-protein kinase transmembrane receptor ROR1 is also known as Neurotrophic tyrosine kinase, receptor-related 1 (NTRKR1), which belongs to the protein kinase superfamily or tyr protein kinase family or ROR subfamily. ROR1 contains 1 FZ (frizzled) domain, 1 Ig-like C2-type (immunoglobulin-like) domain, 1 kringle domain, 1 protein kinase domain. ROR1 is expressed at high levels during early embryonic development. The expression levels drop strongly around day 16 and there are only very low levels in adult tissues. Isoform Short is strongly expressed in fetal and adult CNS and in a variety of human cancers, including those originating from CNS or PNS neuroectoderm. ROR1 could interact with casein kinase 1 epsilon (CK1ε) to activate phosphoinositide 3-kinase-mediated AKT phosphorylation and cAMP-response-element-binding protein (CREB), which was associated with enhanced tumor-cell growth.
Source:
HEK293
Species:
Human
Tag :
His
Predicted N Terminal:
Glu 39
Form:
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4, 10% trehalose.
Molecular Mass:
Human ROR1 (39-151, Ig-like domain), His Tag is fused with a polyhistidine tag at the C-terminus, and has a calculated MW of 13.4 kDa. The reducing (R) protein migrates as 30 kDa in SDS-PAGE due to glycosylation.
Protein length:
AA Glu 39 - Gly 151
Endotoxin:
Less than 1.0 EU per μg of the Human ROR1 (39-151, Ig-like domain), His Tag by the LAL method.
Purity:
>95% as determined by SDS-PAGE.
Storage:
Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4°C); After reconstitution under sterile conditions for 3 months (-70°C).
Reconstitution:
It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4ºC before opening to recover the entire contents.


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Optional requirements on this protein +Expand
C-fusion   N-fusion  Non-tagged
His   GST  Fc  Others
<1.0 eu/μg   <0.1 eu/μg  <0.01 eu/μg  Not required
Monomer Isolation   Dimer Isolation   Not required
>80% by SDS-PAGE   >90% by SDS-PAGE  >95% by SDS-PAGE  Others
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45-1 Ramsey Road, Shirley, NY 11967, USA
USA: 1-631-559-9269  1-631-448-7888
Europe: 44-207-048-3343
FAX: 1-631-938-8127
EMAIL: info@creative-biomart.com

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