| Description : |
This gene encodes a component of the complement system, a part of the innate immune system that plays an important role in inflammation, host homeostasis, and host defense against pathogens. The encoded preproprotein is proteolytically processed to generate multiple protein products, including the C5 alpha chain, C5 beta chain, C5a anaphylatoxin and C5b. The C5 protein is comprised of the C5 alpha and beta chains, which are linked by a disulfide bridge. Cleavage of the alpha chain by a convertase enzyme results in the formation of the C5a anaphylatoxin, which possesses potent spasmogenic and chemotactic activity, and the C5b macromolecular cleavage product, a subunit of the membrane attack complex (MAC). Mutations in this gene cause complement component 5 deficiency, a disease characterized by recurrent bacterial infections. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Nov 2015] |
| Source : |
HEK293 cells |
| Species : |
Human |
| Tag : |
Fc |
| Molecular Mass : |
rhCD100-Fc, fused with Fc fragment Of human IgG1 at the C-terminal and has a calculated MW of 105.4 kDa expressed. The predicted N-terminal is Met 22. Protein migrates as 120-140 kDa in reduced SDS-PAGE due to glycosylation. |
| Endotoxin : |
Less than 1.0 EU per 1 µg of the rhCD100-Fc by the LAL method. |
| Purity : |
>95% as determined by SDS-PAGE. |
| Notes : |
Research Use Only. Not for use in diagnostic or therapeutic procedures. |
| Storage : |
No activity loss was observed after storage at 4-8 centigrade for 1 month or -20 centigrade for 1 year in lyophilized state. rhCD10 centigrade-Fc should be aliquoted and stored at -8 centigrade centigrade in liquid formulation, for long term storage. Avoid repeated freeze-thaw cycles. |
| Storage Buffer : |
Supplied as a 0.22 µm filtered solution in 50 mM tris, 100 mM glycine, pH7.0 and delivered as liquid formulation or lyophilized powder. Normally mannitol or trehalose are added as protectants before lyophilization. Reconstitution instruction or specific concentrations are included in the hardcopy of CoA along with the products. |
