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Active Recombinant Human SIGLEC15 protein, Fc-tagged

Cat.No. : SIGLEC15-15H
Product Overview : Recombinant Human SIGLEC15(Phe20-Thr263) fused with Fc of Human IgG1 tag at C-terminal was expressed in CHO.
  • Specification
  • Gene Information
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Description : Siglec-15 is a transmembrane glycoprotein in the Siglec family of sialic acid-binding immune regulatory molecules. Mature human Siglec-15 consists of a 244 amino acid (aa) extracellular domain (ECD) with two Ig-like domains, a 21 aa transmembrane segment, and a 44 aa cytoplasmic domain. Within the ECD, human Siglec-15 shares 85% aa sequence identity with mouse and rat Siglec-15. Alternative splicing generates an additional isoform that lacks the signal peptide and first Ig-like domain. Siglec-15 associates with the signaling molecules DAP12 and DAP10. It is expressed on osteoclasts, macrophages, and dendritic cells and binds to the sialyl-Tn antigen. This interaction induces the production of TGF-beta by tumor-associated macrophages. Siglec-15 function is important for osteoclast formation and TRANCE/RANK Ligand signaling in osteoclasts.
Source : CHO
Species : Human
Tag : Fc
Predicted N Terminal : Phe20
Form : Lyophilized from a 0.2 μm filtered solution in PBS, NaCl, CHAPS and Trehalose.
Bio-activity : Measured by its binding ability in a functional ELISA. When Neu5Ac(a2-6)GalNAc-PAA-biotin is immobilized onto a Streptavidin-coated plate, Recombinant Human Siglec-15 Fc Chimera binds with an ED50 of 150-900 ng/mL.
Molecular Mass : Predicted Molecular Mass: 53 kDa;
SDS-PAGE: 53-64 kDa, reducing conditions
Protein length : Phe20-Thr263
Endotoxin : <0.10 EU per 1 μg of the protein by the LAL method.
Purity : >90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Storage : Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 centigrade as supplied.
1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
Reconstitution : Reconstitute at 200 μg/mL in water.
Shipping : The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Gene Name : SIGLEC15 sialic acid binding Ig like lectin 15 [ Homo sapiens ]
Official Symbol : SIGLEC15
Synonyms : CD33L3; HsT1361; SIGLEC-15; CD33 antigen-like 3; CD33 molecule-like 3
Gene ID : 284266
mRNA Refseq : NM_213602
Protein Refseq : NP_998767
UniProt ID : Q6ZMC9
Chromosome Location : 18q12.3
Pathway : DAP12 interactions, organism-specific biosystem; Immune System, organism-specific biosystem; Innate Immune System, organism-specific biosystem

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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